Figure 5

Changes in E-cadherin and Snail expression after TPM3 knockdown in HepG2 (A) and SNU-475(B). After TPM3 knockdown, E-cadherin and Snail expression levels were measured by real-time qRT-PCR (top plots). * represents P value < 0.05. X axis represents samples and Y axis represents relative expression ratios of E-cadherin and Snail (siTPM/siNEG). Human GAPDH gene was used as internal control for qRT-PCRs. E-cadherin and Snail expression levels were also measured by western blot (bottom plots). Alpha-tubulin was used as internal control for western blot analysis. siTPM3, siTPM3 transfected HCC cell line; siNEG, negative oligonucleotide transfected HCC cell line. Error bars represent mean ± standard error of mean. * represents P value < 0.05.