Figure 1

TPM3 expression levels in various human HCC cell lines. One normal human liver cell line (THLE-3) and 10 types of HCC cell lines were examined by TPM3-specific real-time qRT-PCR (top plot) and western blot (bottom plot). Human GAPDH gene was used as internal control for qRT-PCR and alpha-tubulin was used as internal control for western blot analysis. In the top plot, X axis represents cell lines and Y axis relative TPM3 expression ratios (tumor/normal). Error bars represent mean ± standard error of mean.