Figure 3

Western blot analysis and immunohistochemical analysis for P-Akt thr308 (A), P-4EBP1 (B) and P-S6 (C) in Bowenoid papulosis and condyloma acuminatum. Western blot validation of immunohistochemical stainings showed a good correlation. Cases b and c which exhibited more than 30% expressions of p-Akt thr308 and p-S6, showed the intensity bands using frozen samples. In contrast, more than 30% expression of p-4EBP1 was detected, with double bands at 15 and 20 kDa in Cases b and c. The expression score indicates 0, >30%; 1, 30% to 60%; 2, >60%. Scale bars = 100 μm.