Lung metastasis in C57BL/6 mice induced from B16F10 tail vein injection. Figure A: Normal lung. Figure B, C and D: A mouse with B16F10 lung metastasis, untreated, vehicle treated or M8 treated, respectively. Figure E: a = normal lung; b = untreated lung with B16F10 metastasis; c = lung with B16F10 metastasis treated with vehicle; d = lung with B16F10 metastasis treated with M8. Treatment caused a statistically significant decrease in metastasis (***P < 0.001) and significantly decreased metastasis as compared with vehicle-treated animals (*P < 0.05). Y axis of graph = transformed mean after normal distribution using the equation to transform data, .