Figure 4
From: XIAP is not required for human tumor cell survival in the absence of an exogenous death signal
XIAP depletion in a panel of tumor cell lines. A. XIAP levels were monitored by Western blot from lysates of untreated cells (unfilled square), electroporated cells, (grey square), cells electroporated with 1 μM control siRNA (dark grey square), or 1 μM s1455 XIAP siRNA (black square) 48 hr following electroporation. XIAP levels were quantified by LICOR Odyssey imaging. Percent XIAP levels are expressed relative to the untreated control for each cell line. B. Viablity of XIAP depleted cells was measured at 24, 48, 72 and 96 hr post electroporation; untreated cells (unfilled square), electroporated cells (light grey square), cells electroporated with 1 μM control siRNA (dark grey square), or 1 μM s1455 XIAP siRNA (black square).