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Table 2 Effects of TNFalpha and Tamoxifen on MCF-7 and MCF-7CLU DNA fragmentation.

From: Effects of clusterin over-expression on metastatic progression and therapy in breast cancer

Percentage Apoptotic Cells

MCF-7

MCF-7CLU

Control

13.6 ± 1.4

7.4 ± 1.2*

Tamoxifen (5 micromolar)

22.2 ± 2.8

5.6 ± 1.4*

TNFalpha (5 ng/mL)

22.6 ± 1.8

6.4 ± 1.8*

  1. MCF-7 and MCF-7 CLU cells were grown in culture and treated with ethanol vehicle, TNFalpha (5 ng/ml) or tamoxifen (5 micromolar) for 48 h as described in Materials and Methods. DNA strand breaks were labeled with Br-dUTP and detected using a FITC-conjugated anti-BrdU antibody and analysed by flow cytometry as described in Materials and Methods. Data are expressed as the percentage of cells positive for DNA fragmentation. Results are expressed as mean ± SD of three independent replicates. (* indicates significant differences between means (MCF-7 versus MCF-7CLU), p < 0.05).