Figure 2

Effect of TNFalpha and tamoxifen on MCF-7 and MCF-7CLU cell adherent cell number. Panel A: MCF-7 (black bars) and MCF-7CLU (grey bars) cells were plated at 20,000 cells per well and allowed to grow for the indicated times. Total adherent cell number was determined by crystal violet staining. Panel B: MCF-7 and MCF-7CLU cells were plated at 10,000 cells per well. Two days later cells were treated with ethanol vehicle or varying concentrations of TNFalpha (1, 5 and 10 ng/ml) for 48 h. Panel C: MCF-7 and MCF-7CLU cells were plated at 10,000 cells per well. Two days later cells were treated with ethanol vehicle or varying concentrations of tamoxifen (1, 5 and 10 micromolar) for 72 h. Data represents mean ± SEM of four values per time point. Similar results were obtained from at least three other independent experiments. *P < 0.05.