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Table 1 Primary antibodies used in the study.

From: In situ protein expression in tumour spheres: development of an immunostaining protocol for confocal microscopy

     Antibody dilution
Targeted antigen Clone name Manufacturer Ab type aFC bpIHC Confocal imaging
Primary antibodies
Ep-CAM HEA-125 Miltenyi-Biotec SAS FITC-mouse IgG1 1:11 - 1:50
CK20 IT-Ks 20.10 Biovalley FITC-mouse IgG1 1:50 - 1:50
Beta-Catenin β-catenin-1 DAKO mouse IgG1 1:20 - 1:50
Beta-Catenin 14/Beta-Catenin BD Biosciences mouse IgG1 - 1:400 -
CA 19-9 C241:5:4:1 Novacastra mouse IgG1 1:20 1:200 1:50
CD133 AC133 Miltenyi-Biotec SAS mouse IgG1 1:11 - 1:50
EBP50 - Calbiochem rabbitcpAb - - 1:300
CD44 G44-26 BD Biosciences mouse IgG2b 1:50 1:10 1:50
ALDH1 44/ALDH BD Biosciences mouse IgG1 - 1:100 1:100
  1. aFC: flow cytometry; bpIHC: paraffin immunochemistry; cpAb: polyclonal antibody