Figure 3

Lovastatin-induced apoptosis in ovarian cancer cells is p53-independent. A: A2780 YFP and A2780 p53DD cells were harvested, lysed, and immunoblotted with anti-p53 antibody to detect p53DD and with anti-tubulin as a loading control. B: A2780 YFP and p53DD cell were seeded and then exposed to 8 GY of radiation for 8 hours, harvested, lysed, and immunoblotted for the detection of p21 and tubulin. C: A2780 pBP and Bcl-2 cells were seeded as above, harvested, lysed, and immunoblotted with antibodies to Bcl-2 and tubulin. All experiments were conducted 2-3 times and representative blots are shown. D: A2780 YFP, p53DD, pBP, and Bcl-2 cells were seeded as above and exposed to 20 μM lovastatin or a control for 48 hours and analyzed by fixed PI to measure the pre-G1 population. Pre-G1 populations are expressed as ratios (lovastatin pre-G1/ethanol pre-G1) and normalized to the YFP vector control (= 1). Data are presented as the mean of 6 independent experiments with error bars representing standard deviation. *, p < 0.05 compared to the YFP control by a Student's t-Test.