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Figure 1 | BMC Cancer

Figure 1

From: Lovastatin induces apoptosis of ovarian cancer cells and synergizes with doxorubicin: potential therapeutic relevance

Figure 1

Lovastatin triggers apoptosis in human ovarian cancer cell lines. A series of experiments were conducted to define the cytotoxic effects of lovastatin on a panel of human ovarian cancer cell lines. A: Cells were exposed to a range of lovastatin concentrations for 48 hours to determine MTT50 values by MTT assay. MTT50s are presented as the mean of 2-4 independent experiments with error bars representing standard deviation. B: Cells were exposed to 20 μM lovastatin or vehicle control for 24 or 48 hours to assess the presence of PARP cleavage by western blotting. Tubulin was probed as a loading control. Experiments were conducted 2-3 times and representative blots are shown (see inset). For fixed PI, cells were seeded and treated as above to 20 μM lovastatin or vehicle control. Results are normalized to the ethanol controls (= 1%) and data are presented as the mean of 2-4 independent experiments with error bars representing standard deviation. C: Cells were seeded as above, exposed to 20 μM lovastatin or a vehicle control for 24 hours, lysed and immunoblotted to detect processed (P) and unprocessed (U) Rap1 and actin. Experiments were conducted 2-3 times and representative blots are shown.

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