A rapid, sensitive, reproducible and cost-effective method for mutation profiling of colon cancer and metastatic lymph nodes

Table 2 Frequency of colon cancer mutations

Mutation	No. Mutated Samples	Frequency in Primary Tumor*	in COSMIC^†	Multiple Mutations^‡
ABL1-F359V	1	0.40%	0/66^§
ABL1 Total	1	0.40%	NF(0/66)^§	100%
AKT1-E17K	1	0.40%	0/31^§
AKT1 Total	1	0.40%	NF(0/31)^§	100%
BRAF-D594V\| G	1	0.40%	NF (0/3179)
BRAF-V600E	28	11.70%	14.60%
BRAF Total	29	12.10%	14.70%	24%
KRAS-G12A	2	0.80%	1.80%
KRAS-G12C	10	4.20%	3.60%
KRAS-G12D	40	16.70%	13.20%
KRAS-G12R	3	1.30%	0.40%
KRAS-G12S	3	1.30%	4.20%
KRAS-G12V	20	8.40%	7.40%
KRAS-G13D	23	9.60%	5.20%
KRAS-A59T	1	0.40%	0.10%
KRAS-Q61L	1	0.40%	0.20%
KRAS-Q61R	1	0.40%	NF (0/1927)
KRAS Total	104	43.50%	36.10%	34%
MET-R970C	2	0.80%	NF (0/77)
MET-T992I	6	2.50%	NF (0/77)
MET Total	8	3.30%	0%	50%
NRAS-G12C	1	0.40%	NF (0/46)
NRAS-G12D	4	1.70%	NF (0/46)
NRAS-G13R	1	0.40%	NF (0/46)
NRAS-G13V	1	0.40%	NF (0/46)
NRAS-Q61H	1	0.40%	NF (0/46)
NRAS-Q61K	1	0.40%	NF (0/46)
NRAS Total	9	3.80%	2.2%	38%
PIK3CA-R88Q	5	2.10%	NF (0/171)
PIK3CA-C420R	2	0.80%	NF (0/171)
PIK3CA-E542K	9	3.80%	4.10%
PIK3CA-E545K	12	5.00%	4.10%
PIK3CA-Q546K	4	1.70%	1.20%
PIK3CA-H701P	1	0.40%	NF (0/171)
PIK3CA-H1047L	1	0.40%	1.80%
PIK3CA-H1047R	14	5.90%	5.30%
PIK3CA Total	48	20.10%	16.40%	80%

*% of C-07 samples with this mutation.
^†Data from COSMIC for colon adenocarcinoma limited to the same mutations interrogated with OncoCarta. The mutations listed are only the ones found in C-07. Some COSMIC amino acid changes are not shown here if they were not mutated in C-07.
^‡% of samples with a mutation in the gene shown and at least one other mutation in C-07 samples.
^§COSMIC data are from large intestine, not specific to colon.

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