Levels of EGFR in SK-OV-3 cells after treatment with YSA-targeted, siRNA-loaded nanogels. EGFR expression in SK-OV-3 cells was determined by immunoblotting after the addition of several concentrations of YSA-targeted, siRNA-loaded nanogels (1 to 1000 μg/mL). All cells were harvested 48 hours after the addition of the siRNA-loaded nanogels. Untargeted but siRNA-loaded nanogels (Ng+siRNA) were included as a control. A change in EGFR expression was not observed between treatment groups as determined by immunoblotting.