Figure 1

NRP-154 cells, but not NRP-152 cells, have constitutively-activated STAT3. NRP-152 and NRP-154 cells were grown in their respective complete media. For IC flow cytometry, confluent cells were harvested, fixed, permeabilized, and stained for P-STAT3, as described in Materials & Methods. Cells were analyzed on a Becton-Dickinson FACScan for fluorescence in FL2. The thin purple line on the histogram shows the results for NRP-152 cells + testosterone; the thick blue line shows the results for NRP-154 cells minus testosterone. The fluorescent staining of NRP-152 cells plus testosterone was the same as for the fluorochrome control on both cell lines, and the same for NRP-152 cells not treated with testosterone (data not shown). The NRP-154 cells were approximately 10 times more fluorescent than the NRP-152 cells (p < 0.001 by Kolmogorov-Smirnov {KS} statistical analysis). A representative histogram from 4 independent experiments is shown here.