Skip to main content

Table 1 Summary of alterations of E-cadherin and β-catenin in a series of 50 gastric tumours.

From: Alterations of E-cadherin and β-catenin in gastric cancer

Tumour Stage Type Grade LOH E-cad gene mutations Aberrant mRNA E-cad β-cat
Sample     at 16q22.1 and polymorphisms of E-cad IHC IHC
1 T3N3 diff   + IVS1+6T→C - +/- ++/-
3 T3N1 squ G1 + IVS4+10C→G ND - +/-
17 T3N1 diff   ND GTG(Val)→GTC(Val) at cd832 ND - -
23   met   + GCC(Ala)→ACC(Thr) at cd592* ND +++/- +++/-
43 T3N2 diff   + - - - +++/-
50 T3N1 diff   - IVS1+6T→C - ++/- ++/-
165 T3N1 int G3 ND - - +++/- +++/-
174 T3N2 int G3 - - ND +++/- ++/-
193 T3N2 int G3 + - ND -/+++ -/++
200 T3N1 int G2 - IVS4+10C→G - ++/- +++/-
231 T3N0 mi G3 + - - ++/- ++/-
283 T3N0 int G3 ND - ND ++/- ++/-
287 T3N1 int G2 + - - -/+ ++/-
294 T3N1M1 mi G4 + GCC(Ala)→ACC(Thr) at cd592♦ - ++/- ++/-
304 T3N3 int G2 - - - -/+++ +/-
308 T3N1 int G2 + - - +++/- ++/-
314 T3N1 diff   - CAC(His)→CAT(His) at cd632 - +++/- ++/-
      GGC(Gly)→GGT(Gly) at cd865    
360 T2N1 int G3 + IVS4+10C→G +♣ ++/- +/-
369   met   + - +♣ +++/- ++/-
433 T3N1 mi G3 - IVS4+10C→G - -/++ -/+
435 T2N0 int G3 + - - -/+++ -/++
443 T2N0 int G4 - - - +++/- +++/-
451 T4N0 int G2 ND - ND +++/- ++/-
474 T3N1 int G3 ND - ND - +/-
493 T3N1 int G3 + - - -/+++ +/-
503 T3N1 int G3 ND - - - +++/-
556 T3N2 int G2 - IVS1+6T→C ND ++/- +/-
      5'UTR-71C→G    
568 T3N2 mi G3 + - +♣ ++/- ++/-
612 T3N0 int G2 ND IVS4+10C→G - +++/- +/-
      AAC(Asn)→AAT(Asn) at cd751    
636 T3N1 diff   + IVS4+10C→G ND +++/- +++/-
650 T2N0M1 int G2 + - ND -/+ ++/-
675 T2N0 mi G3 + IVS4+10C→G - +/- ++/-
676 T3N1 int G2 + - - +++/- -
680 T3N1 mi G3 + IVS1+6T→C - ++/- ++/-
      AAC(Asn)→AAT(Asn) at cd751    
694 T3N1 int G1 + - - +++/- +++/-
717 T3N1 int G3 + - + -/+++ +++/-
726 T2N1 int G2 + IVS4+10C→G - -/+++ ++/-
728 T3N0 int G2 + GCC(Ala)→ACC(Thr) at cd592 - -/+++ ++/-
729 T3N1 int G1 - IVS1+6T→C - -/+++ +++/-
732 T2N1 int G3 + - - -/+++ +++/-
735 T4N3 diff   ND AAC(Asn)→AAT(Asn) at cd751 - +++/- +++/-
738 T3N2 diff   + - - - -
750   met   ND AAC(Asn)→AAT(Asn) at cd751 + ++/- +++/-
755 T2N1 int G2 ND - + +++/- ++/-
808 T3N0 int G1 + ACG(Thr)→ACA(Thr) at cd251 + +++/- ++/-
811 T2N1 int G2 + - - +++/- -/+
832 T3N2 int G2 + - - +++/- +++/-
855 T3N2 int G2 + - - +++/- +++/-
875 T3N2 int G2 - IVS4+10C→G ND +++/- +++/-
904 T2N0 int G3 + IVS4+10C→G - -/+++ ++/-
Useful     30 3 7 21 14
Total     40 50 38 50 50
%     75 6 18 42 28
  1. T, tumour (size and invasiveness); N, node (degree of metastasis); M, metastasis; G1, well differentiated; G2, moderately differentiated; G3, poorly differentiated; G4, not differentiated; diff, diffuse (degree of differentiation=G4); mi, mixed; int, intestine; met, metastatic tumour probably from stomach tumour; squ, squamous epithelial; LOH, loss of heterozygosity; E-cad, E-cadherin; β-cat, β-catenin; IHC, immunohistochemistry; cd, codon; UTR, untranslated region; +, positive LOH, aberrant E-cad mRNA, E-cad and β-cat IHC;-, negative LOH, E-cadherin gene mutation, aberrant E-cad mRNA, E-cad and β-cat IHC; ND, not determined or not done; +/-, ++/- and +++/-, more than 50% cells positive; -/+, -/++ and -/+++, more than 50% cells negative; *, somatic mutation; ♦, germline mutation; ♣, insertion of intron 7 between exons 7 and 8, stop codon 374; ♥, deletion of last 72 bases of exon 7, exon 8 and first 124 bases of exon 9, stop codon 322; ♠, deletion of exons 8 and 9; deletion of last 84 bases of exon 8, stop codon 358; , these samples also showed cytoplasmic staining for β-catenin IHC.
\