Development and validation of a simple questionnaire for the identification of hereditary breast cancer in primary care
- Patricia Ashton-Prolla1, 2, 3, 4, 5, 6, 7Email author,
- Juliana Giacomazzi2, 3, 4, 6,
- Aishameriane V Schmidt8,
- Fernanda L Roth3, 6,
- Edenir I Palmero1, 2, 3,
- Luciane Kalakun3, 9,
- Ernestina S Aguiar2, 3, 4, 6,
- Susana M Moreira3, 7,
- Erica Batassini3, 9,
- Vanessa Belo-Reyes3, 6,
- Lavinia Schuler-Faccini1, 4, 5, 7,
- Roberto Giugliani1, 4, 5, 6, 7,
- Maira Caleffi3 and
- Suzi Alves Camey9, 10
© Ashton-Prolla et al; licensee BioMed Central Ltd. 2009
Received: 19 January 2009
Accepted: 14 August 2009
Published: 14 August 2009
Breast cancer is a significant public health problem worldwide and the development of tools to identify individuals at-risk for hereditary breast cancer syndromes, where specific interventions can be proposed to reduce risk, has become increasingly relevant. A previous study in Southern Brazil has shown that a family history suggestive of these syndromes may be prevalent at the primary care level. Development of a simple and sensitive instrument, easily applicable in primary care units, would be particularly helpful in underserved communities in which identification and referral of high-risk individuals is difficult.
A simple 7-question instrument about family history of breast, ovarian and colorectal cancer, FHS-7, was developed to screen for individuals with an increased risk for hereditary breast cancer syndromes. FHS-7 was applied to 9218 women during routine visits to primary care units in Southern Brazil. Two consecutive samples of 885 women and 910 women who answered positively to at least one question and negatively to all questions were included, respectively. The sensitivity, specificity and positive and negative predictive values were determined.
Of the 885 women reporting a positive family history, 211 (23.8%; CI95%: 21.5–26.2) had a pedigree suggestive of a hereditary breast and/or breast and colorectal cancer syndrome. Using as cut point one positive answer, the sensitivity and specificity of the instrument were 87.6% and 56.4%, respectively. Concordance between answers in two different applications was given by a intra-class correlation (ICC) of 0.84 for at least one positive answer. Temporal stability of the instrument was adequate (ICC = 0.65).
A simple instrument for the identification of the most common hereditary breast cancer syndrome phenotypes, showing good specificity and temporal stability was developed and could be used as a screening tool in primary care to refer at-risk individuals for genetic evaluations.
According to the World Cancer Report, cancer rates will increase by 50% from 10 million new cases estimated for the year 2000 to 15 million new cases per year in 2020. However, the report also provides evidence that efforts in the control of risk factors and public health strategies to increase surveillance and thus promote early cancer detection could prevent as many as one third of cancers diagnosed in the world [1, 2].
Among women, breast cancer (BC) is the most prevalent malignant tumor, and one in four cancers diagnosed in women worldwide is a cancer of the female breast. More than 1.1 million women are diagnosed each year with the disease and its incidence rates are still increasing in many countries [2, 3]. In Brazil, BC is a significant public health problem, due to its morbidity, and high incidence and mortality rates. About half of the affected women are diagnosed in advanced stages and not surprisingly, mortality rates are still increasing [4, 5]. The State of Rio Grande do Sul (RS) has one of the highest BC incidence rates of the country with a predicted rate of 85.50 per 100,000 estimated for the year of 2008 – which is comparable to the USA and North Europe. The State's capital, Porto Alegre, has an even higher BC incidence rate, with 119.72 new cases per 100,000 women .
Positive family histories of BC and other tumors are associated with an increased risk for developing the disease and are recognized as indicators for the identification of high-risk, genetically predisposed individuals [6, 7]. Overall, an estimated 5–10% of all breast cancers is hereditary, i.e., caused by a germline mutation in a predisposition gene that confers to its carrier a significantly higher cancer risk. Data on the prevalence of hereditary breast cancer in Brazil is scarce, and although founder mutations in cancer predisposition genes have been described in the country, there is no evidence for increased frequency of hereditary cancer syndromes in the Brazilian population [, Garritano S, Gemignani F, Palmero EI, Olivier M, Martel-Planche G, Calvez-Kelm FL, Brugières L, Vargas FR, Brentani RR, Ashton-Prolla P, Landi S, Tavtigian SV, Hainaut P, Achatz MI. High frequency of the cancer-predisposing TP53 mutation p.R337H in the population of Southern Brazil: evidence for a founder effect, submitted to Human Mutation]. Germline mutations in the BRCA1 and BRCA2 genes are related to an increased risk for breast, ovarian and other cancers in the autosomal dominant Hereditary Breast and Ovarian Cancer (HBOC) syndrome [9, 10]. Clinically significant BRCA mutations are estimated to occur in 1 in 300 to 500 persons in the general population . Other BC predisposition genes, such as TP53 (associated with Li-Fraumeni and Li-Fraumeni-like syndromes, LFS/LFL), PTEN (associated with Cowden syndrome, CS) and CHEK2 (associated with Hereditary Breast and Colorectal Cancer, HBCC) have been identified and are thought to have important, albeit lower contributions to hereditary breast cancer (HBC) [12–17]. Specific features in the family history may suggest the diagnosis of a hereditary breast cancer syndrome (HBCS) (i.e. pre-menopausal BC, male BC, bilateral BC and family history of BC and ovarian cancer – OC) [9, 11].
Taking a family history has long been considered an integral part of the medical evaluation and is particularly important in genetic risk evaluation and risk management protocols, where it may ultimately help in the design of strategies to reduce cancer-associated mortality . Health care providers have a professional and legal duty to obtain sufficient family history information to perform adequate cancer risk assessments [19, 20]. However, despite its importance, information about the family history is not routinely or sufficiently collected outside the setting of cancer risk evaluation programs; in primary care, it has been described as a neglected area [21, 22]. In addition, even among highly educated women, knowledge of HBC risk factors that could influence self-referral is usually scarce 
In Brazil, several barriers to the identification and counseling of individuals at-risk for HBCS have been identified and include lack of well established cancer genetic counseling services, absence of specific training programs in cancer genetics, small numbers of certified clinical geneticists and their unequal geographic distribution in the country . The genetic cancer risk assessment process is an activity that requires specific training, in-depth knowledge of the subject, a significant amount of time and a multidisciplinary approach. Although little is known about the efficacy and the cost-benefit relationship of community-based programs of identification of individuals at-risk for hereditary cancer, training of primary health care professionals and use of simple tools to facilitate the identification of these individuals may be helpful to ensure proper referrals and to optimize evaluations, especially in low resource countries [24, 25].
In this study, a simple instrument to inquire about the family history of BC, OC and CRC was developed to identify women at-risk for HBCS during their primary care visits. We tested the instrument's sensitivity and positive and negative predictive values to identify different HBC phenotypes and its ability to identify women with higher lifetime risks for developing BC as estimated by current risk models.
Development of an instrument to identify women at risk for HBC in primary care
In April of 2004, a population-based cohort study (the Núcleo Mama Porto Alegre – NMPOA Cohort) was started in 18 primary care units of the city of Porto Alegre, Southern Brazil [26, 27]. The purpose was to collect demographic and epidemiologic data of a large sample of women and test a model for community-based BC screening in an underserved population that relies upon the Programa Saúde da Família (PSF or Family Health Program). This program was created in the mid-90's and is based upon multidisciplinary teams, composed by a physician, nurse, one or two nurse assistants and 4–6 lay community health workers. This team provides primary health care to a geographically defined group of approximately 600 families. The Programa Saúde da Família has expanded rapidly and nowadays provides health care to about half of the population [28–30].
The FHS-7 questionnaire: intra-class correlation (ICC) between responses to individual questions in two settings: primary care unit (PCU) and immediately before genetic cancer risk assessment (GCRA) and total of positive answers at GCRA interview.
Did any of your 1st degree relatives have breast or ovarian cancer?
Did any of your relatives have bilateral breast cancer?
Did any man in your family have breast cancer?
Did any woman in your family have breast and ovarian cancer?
Did any woman in your family have breast cancer before the age of 50 years?
Do you have 2 or more relatives with breast and/or ovarian cancer?
Do you have 2 or more relatives with breast and/or bowel cancer?
Patient recruitment and definition of study groups
Genetic Cancer Risk Assessment (GCRA)
Genetic evaluation included medical and family histories recorded in detailed pedigrees with information traced as far backwards and laterally as possible, extending to paternal lines and including a minimum of three generations using standard methods. Confirmation of the cancer family history was attempted in all cases and pathology reports, medical records and/or death certificates were obtained whenever possible.
a) Lifetime breast cancer risk estimates (LBCRE): were obtained using the Claus Tables and the Gail and Tyrer-Cuzick models [31–33]. Upper age limit considered for the three LBCRE is 79, 90 and 80 years, respectively.
b) Patients at high-risk for a hereditary breast cancer syndrome (HBC): all patients fulfilling criteria for a BC predisposition syndrome upon pedigree analysis were classified in this group. Criteria for the definition of HBC syndromes are detailed below. For the HBOC syndromes, the American Society of Clinical Oncology (ASCO) criteria were used [35, 36]. In addition, prior probabilities of carrying a BRCA1 or BRCA2 mutation were determined for each patient using mutation prevalence tables and the Penn II mutation prediction model [35–37]. All pedigrees were reviewed by at least two clinical geneticists to assess presence of criteria for Li-Frameni syndrome, Li-Fraumeni-Like syndrome, HBCC or other cancer predisposition syndromes. For Li-Fraumeni syndrome, the original criteria described by Li and Fraumeni  were used; for Li-Fraumeni-Like syndrome, pedigrees were classified according to the criteria of Birch  and Eeles  and for HBCC, the Meijers-Heijboer and Nasseem criteria were used [15–38].
Intra-class correlation (ICC) were used to verify the agreement between results obtained by the instrument and the genetic cancer risk evaluation. In addition, ICC was used to measure agreement between answers to the instrument when applied in two different occasions, for temporal stability verification.
To determine the cutoff value for the original FHS-7 instrument, the ROC curve was used and the values for specificity and sensitivity were calculated. To compare the mean risk estimates obtained using the Gail, Claus and Tyrer-Cuzick models, t test for different samples was applied. In all analyses a significance level of 0.05 was considered and two-sided analyses were performed.
Demographics and variables of the women enrolled in this study.
Women reporting a FH1 of cancer in PCU2 (n = 885)
Women without FH1 report of cancer in PCU2 (n = 910)
Age at assessment
Age at menarche
One or more children
Age at birth of first child
Age at menopause
Hormone replacement therapy
After application of FHS-7, 885 women responded affirmatively to at least one question: 375 women (42.4%) reported a first-degree family history of BC or OC, 109 (12.3%), bilateral BC, 10 (1.1%) male BC, 40 (4.5%) BC and OC double primary tumors, 557 (62.9%), BC under the age of 50 years, 222 (25.1%), ≥ 2 relatives with BC and/or OC and 222 (26.2%) ≥ 2 relatives with BC and/or CRC in their families.
Total number of women fulfilling different criteria for hereditary breast cancer syndromes in both study groups.
Women reporting a FH1 of cancer in PCU2 (n = 885)
Women with a negative FH1 for cancer in PCU2 (n = 910)
ASCO criteria for HBOC syndrome3
High risk HBOC syndrome4
HBC syndrome (overall)5
Regarding temporal stability of the instrument, ICC for each of the 7 questions were: 0.72 for BC or OC in a first-degree relative, 0.74 for BC diagnosed <50 years in a relative, 0.61 for ≥ 2 relatives with BC and/or OC, 0.38 for bilateral BC in a relative, 0.17 multiple BC and OC in a relative and 0.47 for ≥ 2 relatives with BC and/or CRC in the family. For the question about multiple BC and OC in a relative there were only 5 positive answers in GCRA. For the male BC question there was no positive answer and thus, the ICC coefficient could not be calculated. When considering the instrument as a whole, the presence of at least one positive answer in both instrument applications had an ICC of 0.65.
Ability of the FHS-7 to identify women with clinical criteria (family history) for hereditary breast cancer syndromes (n = 1795).
AUC (CI 95%)
Cut point = 1
Sensitivity (CI 95%)
Specificity (CI 95%)
High risk HBOC syndrome4
HBC syndrome (overall)5
Although the family history of cancer is the single most important tool for the initial identification of hereditary cancer syndromes and usually does not require any sophisticated technologies, little attention and time is usually spent to obtain a detailed pedigree in routine clinical practice. Even in tertiary health care institutions, recording of a comprehensive pedigree is uncommon. Most health care professionals outside clinical genetics are not trained to obtain a detailed family history, usually do not have sufficient time and do not recognize the power of this simple tool for disease identification and prevention . In fact, reported family history of cancer, especially when in first-degree relatives appears to be quite reliable, and such reliability may exist regardless of educational level of the informant . Since most at-risk families are identified first by a primary health care professional, development of simple tools to facilitate the identification of such families at the primary care level can be useful and effective to optimize referrals [40, 41], especially in low resource countries. In this study, we developed and validated a simple family history questionnaire, FHS-7, for application at the primary care level and used a cut point of at least one positive question to determine referral for genetic evaluation by a trained specialist.
Overall, in the process of instrument validation, most of the women responding positively to at least one of the seven questions at the primary care units had also at least one positive finding regarding the same seven family history features identified during GCRA by a trained clinical geneticist. This indicates that there is a good agreement between the information obtained with the instrument in primary care and the information obtained during GCRA. For some of the questions, such as those inquiring about a family history of male BC and multiple primary (breast and ovarian) tumors, the ICC was very low and this may be a consequence of the small number of positive answers obtained for these questions. An additional reason that may partially explain some of the low ICC values is that upon retrieval of confirmatory documents during GCRA, some of the cancer diagnoses were not confirmed. This occurred particularly often in the multiple primary cases, and in those, OC was usually not confirmed. Uncertainty about the cancer family history, especially when there is more than one cancer diagnosis in the family, may also be frequent at the first visit in the primary care unit and may be confirmed or refuted before the genetic evaluation. Another concern would be a potential bias of ascertainment due to non-inclusion of women who did not attend genetic cancer risk assessment sessions. In fact, the demographic data of women with a positive family history who underwent GCRA (attenders) differed significantly in some aspects from those of women who did not undergo GCRA (non-attenders). Non-attenders were generally younger, less educated, and had undergone a breast biopsy less often than attenders (p < 0.001), as demonstrated elsewhere. However, for most of the questions in the family history questionnaire, there was no significant difference between the proportion of positive answers between attenders and non-attenders .
Regarding the ability of FHS-7 to identify particular HBC phenotypes, the presence of at least one positive answer was associated with good sensitivity at acceptable specificity. This was particularly the case for the identification of the HBOC phenotype, and less for the Li-Fraumeni-Like phenotype, however, the instrument was not originally developed to identify Li-Fraumeni-Like families. The worst performance was in the identification of Li-Fraumeni-Like families and all of them were defined as Li-Fraumeni-Like by Eeles criteria only . These findings indicate that individuals with family history features suggesting Li-Fraumeni syndrome variants, especially those fulfilling the more relaxed criteria of Eeles may be missed by the instrument. Since the major goal of using the instrument was to correctly identify HBC phenotypes in general, the very high negative predictive values obtained considering cut point 1 was highly satisfactory, even if this required evaluation of a larger number of individuals to ensure that only a very small fraction of high-risk cases remain unidentified. The relatively low specificity of FHS-7 must be considered as a potential limitation for this study. However, if one considers the context of the study, including the low cost involved in the application of the instrument as well as of clinical genetic evaluations, and the added significant benefit of identification of a true hereditary breast cancer family, in which several at-risk individuals may benefit from further genetic testing and screening interventions, the low specificity becomes less important. An additional limitation of the study is that we have not considered limited family structure in the pedigree analyses. Weitzel et al. (2007)  have recently described an increased prevalence of germline mutations in women diagnosed with early-onset breast cancer, who may not fulfill well-established criteria for a hereditary breast cancer syndrome because of the lack of females in either lineage. If this would have been considered, prevalence of phenotype-positive cases would have likely increased.
A significant proportion of women from the community-based sample studied here presented a family history of BC, OC and/or CRC and upon genetic risk evaluations: 6.2% of them had phenotypes of specific BC predisposition syndromes. These results have been reported elsewhere in detail in . Even considering that the majority of families with family histories of a hereditary breast cancer syndrome fulfilled the more relaxed criteria for Li-Fraumeni-like syndrome, the identification of such families may still be relevant since this geographic has been identified as a region with high prevalence of a specific TP53 germline mutation.
In conclusion, simple family history questionnaires such as the one developed here can be used in BC risk-screening programs at the primary care level as important tools for the identification of individuals who may benefit from specific interventions.
The Núcleo Mama Porto Alegre – NMPOA Cohort, from which the patients derive, is maintained by Associação Hospitalar Moinhos de Vento, in a partnership with Instituto da Mama do Rio Grande do Sul and the Municipal Health Agency from Porto Alegre. The authors are indebted to Giovana Skonieski, Karen Barboza de Pereira, Diego Pasetto, and the NMPOA team for their help with the recruitment, evaluation and follow-up of the patients included in this study. This study was supported by a grant from Susan G. Komen for the Cure (POP0403033), and in part by grants from Fundo de Incentivo à Pesquisa -FIPE, Hospital de Clínicas de Porto Alegre (# 04-170), FAPERGS (grant number PPSUS-II 0700964) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES – PRODOC grant number 00202/03-7) to P.A-P. EIP was supported in part by grants from The International Agency for Research in Cancer (IARC) and CNPq (process number 203732/2005-7); JG was supported by a fellowship from CAPES and AVS received an undergraduate grant from the Federal University of Rio Grande do Sul, Brazil (PIBIC, Propesq-UFRGS).
- World Health Organization Webpage. [http://www.who.int]
- Cancer Research UK. [http://info.cancerresearchuk.org/cancerstats/geographic/world/commoncancers/]
- Tavassoli FA, Devilee P, (Eds.): World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of the Breast and Female Genital Organs. 2003, IARC Press: IARC Press: Lyon
- Brazilian National Cancer Institute. [http://www.inca.gov.br]
- Cadaval Gonçalves AT, Costa Jobim PF, Vanacor R, et al: Increase in breast cancer mortality in Southern Brazil from 1980 to 2002. Cad Saúde Pública. 2007, 23: 1785-1790.View ArticlePubMedGoogle Scholar
- Hall IJ, Burke W, Coughlin , et al: Population-Based Estimates of the Prevalence of Family History of Cancer Among Women. Community Genet. 2001, 4: 134-142. 10.1159/000051173.PubMedGoogle Scholar
- Eberl MD, Sunga A, Farrel C, et al: Patients with a family history of cancer: identification and management. J Am Board Fam Medicine. 2005, 18: 211-217. 10.3122/jabfm.18.3.211.View ArticleGoogle Scholar
- Da Costa EC, Vargas FR, Moreira AS, et al: Founder effect of the BRCA1 5382insC mutation in Brazilian patients with hereditary breast ovary cancer syndrome. Cancer Genet Cytogenet. 2008, 184: 62-6. 10.1016/j.cancergencyto.2008.03.011.View ArticlePubMedGoogle Scholar
- Garber JE, Offit K: Hereditary cancer predisposition syndromes. J Clin Oncol. 2005, 23: 276-292. 10.1200/JCO.2005.10.042.View ArticlePubMedGoogle Scholar
- Online Mendelian Inheritance in Man Webpage. [http://www.ncbi.nlm.nih.gov/sites/entrez?db=OMIM&cmd=search&term]
- Nelson HD, Huffman LH, Fu R, et al: U.S. Preventive Services Task Force Genetic Risk Assessment and BRCA Mutation Testing for Breast and Ovarian Cancer Susceptibility: Systematic Evidence Review for the U.S. Preventive Services Task Force. Ann Intern Med. 2005, 143: 362-379.View ArticlePubMedGoogle Scholar
- Li FP, Fraumeni JF, Mulvihill JJ, et al: A cancer family syndrome in twenty-four kindreds. Cancer Res. 1988, 48: 5358-5362.PubMedGoogle Scholar
- Birch JM, Hartley AL, Tricker KJ, et al: Prevalence and diversity of constitutional mutations in the p53 gene among Li-Fraumeni families. Cancer Res. 1994, 54: 1298-1304.PubMedGoogle Scholar
- Eeles RA: Germline mutations in the TP53 gene. Cancer Surv. 1995, 25: 101-124.PubMedGoogle Scholar
- Meijers-Heijboer H, Wijnen J, Vasen H, et al: The CHEK2 1100delC mutation identifies families with a hereditary breast and colorectal cancer phenotype. Am J Hum Gen. 2003, 72: 1308-1314. 10.1086/375121.View ArticleGoogle Scholar
- Vahteristo P, Bartkova J, Eerola H, et al: A CHEK2 genetic variant contributing to a substantial fraction of familial breast cancer. Am J Hum Genet. 2002, 71: 432-438. 10.1086/341943.View ArticlePubMedPubMed CentralGoogle Scholar
- Eng C: The real Cowden syndrome please stand up: revised diagnostic criteria. J Med Genet. 2000, 37: 828-30. 10.1136/jmg.37.11.828.View ArticlePubMedPubMed CentralGoogle Scholar
- Allen HJ, Briceno CA, Piver MS: Assessment of family cancer history collection and utilization in patient care. Community Genet. 1998, 1: 237-242. 10.1159/000016169.PubMedGoogle Scholar
- Murff HJ, Byrne D, Syngal S: Cancer Risk Assessment: Quality and Impact of the Family History Interview. Am J Prev Medicine. 2004, 27: 239-245.Google Scholar
- Offit K, Groeger E, Turner S, et al: The "duty to warn" a patient's family members about hereditary disease risks. JAMA. 2004, 292: 1469-1473. 10.1001/jama.292.12.1469.View ArticlePubMedGoogle Scholar
- Roth FL, Camey SA, Caleffi M, et al: Consistency of self-reported first-degree family history of cancer: a population-based study. Familial Cancer. 2009,Google Scholar
- Ramsey SD, Yoon P, Moonesinghe , et al: Population-based study of the prevalence of family history of cancer: Implications for cancer screening and prevention. Genet Med. 2006, 8: 571-575.View ArticlePubMedPubMed CentralGoogle Scholar
- Katapodi MC, Aouizerat BE: Do women in the community recognize hereditary and sporadic breast cancer risk factors?. Oncol Nurs Forum. 2005, 32: 617-623. 10.1188/05.ONF.617-623.View ArticlePubMedGoogle Scholar
- Palmero EI, Ashton-Prolla P, da Rocha JC, et al: Clinical characterization and risk profile of individuals seeking genetic counseling for hereditary breast cancer in Brazil. J Genet Couns. 2007, 16: 363-371. 10.1007/s10897-006-9073-0.View ArticlePubMedGoogle Scholar
- Daly MB, Stearmen B, Masny A, et al: How to establish a high-risk cancer genetics clinic: limitations and successes. Curr Oncol Rep. 2005, 7: 469-474. 10.1007/s11912-005-0012-2.View ArticlePubMedGoogle Scholar
- Caleffi M, Ribeiro RA, Duarte Filho DL, et al: A model to optimize public health care and downstage breast cancer in limited-resource populations in southern Brazil. (Porto Alegre Breast Health Intervention Cohort). BMC Public Health. 2009, 9: 83-10.1186/1471-2458-9-83.View ArticlePubMedPubMed CentralGoogle Scholar
- Palmero EI, Caleffi M, Schüler-Faccini L, et al: Population prevalence of hereditary breast cancer phenotypes and implementation of a genetic cancer risk assessment program in Southern Brazil. Accepted in Genetics and Molecular Biology. 2009, 32:Google Scholar
- Ramalho AS, Silva RB: Community genetics: a new discipline and its application in Brazil. Cad Saude Publica. 2000, 16: 261-263. 10.1590/S0102-311X2000000100029.View ArticlePubMedGoogle Scholar
- Ministério da Saúde, Brasil. Secretaria de Assistência à Saúde. Instituto Nacional do Câncer: Câncer no Brasil: Dados dos Registros de Base Populacional. Rio de Janeiro. 2003, 125-Google Scholar
- Harzheim E, Duncan BB, Stein AT, et al: Quality and effectiveness of different approaches to primary care delivery in Brazil. BMC Health Serv Res. 2006, 6: 156-10.1186/1472-6963-6-156.View ArticlePubMedPubMed CentralGoogle Scholar
- Gail MH, Brinton LA, Byar DP, et al: Projecting individualized probabilities of developing breast cancer for white females who are being examined anually. J Natl Cancer Inst. 1998, 81: 1879-1886. 10.1093/jnci/81.24.1879.View ArticleGoogle Scholar
- Claus EB, Risch N, Thompson D: Autosomal dominant inheritance of early-onset breast cancer. Implications for risk prediction. Cancer. 1994, 73: 643-651. 10.1002/1097-0142(19940201)73:3<643::AID-CNCR2820730323>3.0.CO;2-5.View ArticlePubMedGoogle Scholar
- Tyrer J, Duffy SW, Cuzick J: A breast cancer prediction model incorporating familial and personal risk factors. Stat Med. 2005, 23: 1111-1130. 10.1002/sim.1668.View ArticleGoogle Scholar
- National Comprehensive Cancer Network. Genetic/Familial High-Risk Assessment: Breast and Ovarian. Clinical Practice Guidelines in Oncology. 2005, 24: 156-[http://www.nccn.org/professionals/physician_gls/PDF/genetics_screening.pdf]Google Scholar
- ASCO Subcommittee on Genetic Testing for Cancer Susceptibility: Statement of the American Society of Clinical Oncology: Genetic Testing for Cancer Susceptibility. J Clin Oncol. 2006, 14: 1730-1736.Google Scholar
- Penn II Mutation Prediction Model. [http://www.afcri.upenn.edu/itacc/penn2/]
- Parmigiani G, Chen S, Iversen ES, et al: Validity of models for predicting BRCA1 and BRCA2 mutations. Ann Intern Med. 2007, 147: 441-450.View ArticlePubMedPubMed CentralGoogle Scholar
- Naseem H, Boylan J, Speake D, et al: Inherited association of breast and colorectal cancer: limited role of CHEK2 compared with high-penetrance genes. Clin Genet. 2006, 70: 388-395. 10.1111/j.1399-0004.2006.00698.x.View ArticlePubMedGoogle Scholar
- Penchaszadeh VB: Community genetics in Latin America: challenges and perspectives. Community Genet. 2000, 3: 124-127. 10.1159/000051121.Google Scholar
- Hughes KS, Roche C, Campbell CT, et al: Prevalence of family history of breast and ovarian cancer in a single primary care practice using a self-administered questionnaire. Breast J. 2003, 1: 19-25. 10.1046/j.1524-4741.2003.09105.x.View ArticleGoogle Scholar
- Hoskins KF, Zwaagstra A, Ranz M: Validation of a tool for identifying women at high risk for hereditary breast cancer in population-based screening. Cancer. 2006, 107: 1769-1776. 10.1002/cncr.22202.View ArticlePubMedGoogle Scholar
- Gonzalez KD, Noltner KA, Buzin CH, et al: Beyond Li Fraumeni Syndrome: clinical characteristics of families with p53 germline mutations. J Clin Oncol. 2009, 27: 1250-1256. 10.1200/JCO.2008.16.6959.View ArticlePubMedGoogle Scholar
- Weitzel JN, Lagos VI, Cullinane CA, et al: Limited Family Structure and BRCA Gene Mutation Status in Single Cases of Breast Cancer. JAMA. 2007, 297: 2587-2595. 10.1001/jama.297.23.2587.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/9/283/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.