A novel approach to simultaneously scan genes at fragile sites
© Willem et al; licensee BioMed Central Ltd. 2006
Received: 23 March 2006
Accepted: 08 August 2006
Published: 08 August 2006
Fragile sites are regions of the genome sensitive to replication stress and to exposure to environmental carcinogens. The two most commonly expressed fragile sites FRA3B and FRA16D host the histidine triad (FHIT) and WW domain containing oxidoreductase (WWOX) genes respectively. There is growing evidence that both genes contribute to cancer development and they are frequently altered by allelic and homozygous deletions in a variety of tumors. Their status is linked to prognosis in several malignancies and they are thought to be involved in early tumorigenesis.
The loci for FHIT and WWOX both span over a megabase but the genes encode for small transcripts. Thus the screening of intragenic deletion can be difficult and has relied on loss of heterozygosity LOH assays, or genomic arrays.
Multiplex ligation dependent probe amplification MLPA, allows for the detection of deletions/duplications and relative quantification of up to 40 specific probes in a single assay. A FHIT/WWOX MLPA assay was designed, applied and validated in five esophageal squamous cell carcinoma ESCC, cell lines established in South Africa where this cancer is of high prevalence. Sixteen probes covered all FHIT exons and 7 probes covered WWOX.
Both homozygous and hemizygous deletions were detected in FHIT, in four of the cell lines with a preferential deletion of exons 5 and 4. Chromosome 3 short arm was present in normal copy number indicating that deletions were site specific. In contrast WWOX was not altered in any cell lines. RT-PCR expression pattern paralleled the pattern of deletions. Ten primary ESCC tumor specimens were subsequently screened with this assay. FHIT exon deletions were found in four of them.
This method offers an alternative to loss of heterozygosity studies. Simultaneous scanning of FHIT and WWOX exons in the context of early tumorigenesis and tumor progression, may help clarify the mechanistic events related to cancer development which are not revealed by imuno histochemistry assays. The presence of site specific deletions of FHIT in these cell lines and primary tumors support its possible role in South African ESCC and justifies a wider screening.
The two most commonly expressed human fragile sites, FRA3B and FRA16D, harbor genes which have a tumor suppressive function, namely the fragile histidine triad, FHIT, and the WW domain containing oxidoreductase, WWOX genes respectively [1–3]. Both genes have small exons distributed over their respective fragile loci and large intragenic deletions have been detected in a wide variety of malignant and pre-malignant tumors, reviewed in [4, 5]. In particular, FHIT inactivation can occur in early stages of carcinogenesis , and also correlates significantly with malignant progression [7–9]. Both WWOX and FHIT have been implicated in cancers that are strongly associated with environmental carcinogens such as smoking and alcohol consumption.
Esophageal squamous cell carcinoma ESCC is a common cancer in South Africa, ranking as second most common malignancy in black males and third most common in black females . Similarly to other parts of the world where this cancer is of high prevalence, a strong association between environmental exposure and the risk of developing ESCC has been demonstrated. Risk factors include heavy smoking , exposure to fumonisin, a fungal toxin produced by fusarium fungi growing on local maize , consumption of alcohol, and home made beer fermented from infected maize [13, 14]. Human Papilloma virus HPV infection, and poor nutrition, have been made to make a contribution to the development of ESCC .
The above risk factors and carcinogens have the potential to affect the integrity of fragile sites directly or indirectly. Tobacco exposure increases the expression of common fragile sites , HPV preferentially integrates within fragile sites loci , alcohol and fumonisin both affect folate intake which may facilitate the expression of fragile sites [18–20], and fumonisin exposure in cell cultures increases the incidence of chromosomal damage [21, 22]. This strengthens the hypothesis that the combinatorial effect of all or some of these factors may have a role in the initiation of genetic instability early in the disease and that fragile sites may be early targets. At present the genes within the most common fragile sites FHIT and WWOX have not been examined in South African ESCC.
The most commonly used method to investigate deletions in FHIT and WWOX is loss of heterozygosity (LOH) analysis in separate assays [23–25]. Other methods such as immuno histochemical (IHC) assays have provided a considerable amount of data regarding the loss of Fhit protein in a variety of cancers [26–29], IHC however does not inform on the nature of inactivating genetic events that may reflect the role of etiological factors. RT-PCR studies are dependent on the availability of fresh material, which are often difficult to obtain, as most biopsy specimens are very small or fixed in paraffin. Since genomic deletions and hypermethylation appear to be the main mechanism of FHIT and WWOX inactivation [6, 9, 30, 31] a significant amount of information can be retrieved from the retrospective evaluation of archived paraffin embedded specimens.
Although the FHIT locus spans 1,67 Mb and WWOX spans more than 750 kb [2, 32], both genes only code for transcripts of around 1 kb. It should be noted that the detection of LOH in the respective fragile locus does not always reflect the loss of coding exons or of protein product . In addition, deletions within fragile sites may be heterogeneous within one cell line, which could bias LOH results.
In order to obtain an exon specific, cost effective and high throughput method to screen ESCC specimens for FHIT and WWOX genomic deletions, a new assay was designed using the existing multiplex ligation-dependent probe amplification MLPA technology . This assay allowed for the detection of deletions/duplications and relative quantification of both FHIT and WWOX exons in a single run. We evaluated its performance investigating FHIT and WWOX deletions in five ESCC South African established cell lines. The assay was subsequently used to screen the genomic status of these two genes in ten primary ESCC tumors.
The five esophageal carcinoma cell lines investigated here were originally established from black South African patients with known esophageal squamous cell carcinoma. These were previously described in the literature and referred to as cell lines: SNO, WHCO1, WHCO3, WHCO5 and WHCO6, [34, 35]. Cells were grown in Dulbecco Modified Eagles medium (DMEM): HAMS F12 (GIBCO) (3:1) supplemented with 10% fetal calf serum (FCS) in a humidified atmosphere at 37°C.
ESCC primary tumor tissue samples
Endoscopic esophageal tumor biopsies were collected from ESCC patients by a gastroenterologist in the course of routine diagnostic investigations. Touch preparations were prepared from each sample for histological assessment and were examined by an experienced pathologist.
The research was approved by the University of the Witwatersrand Ethical Committee and patients were enrolled after written consent was obtained.
After trypsinisation and centrifugation of the cultured ESCC cells, DNA was extracted by standard phenol:chloroform extraction followed by ethanol precipitation. DNA from tumor biopsies was extracted by the same method.
RNA was extracted from each cell line using the Qiagen RNeasy kit (Qiagen GmbH, Hilden Germany) according to the manufacturer's instructions. RT-PCR for the FHIT full transcript was performed using previously published primer sequences . Primers were designed for WWOX full transcript analysis. They were: forward primer 5'-GAG TTC CTG AGC GAG TGG-3' mapping in exon 1, and reverse: 5'-GCT CGT TGG AGA AGA GGA-3' mapping in exon 9.
Fluorescence in-situhybridization, FISH
Metaphases from each cell line were prepared as per standard cytogenetic techniques. FISH analysis using probes specific for the short arm of chromosome 3 and the long arm of chromosome 16 (Qbiogene, Strasbourg, France) was performed as per manufacturer's protocols (Universal FISH protocol).
The MLPA method initially developed and described by Schouten et al,  was used to detect exons specific copy number change in the FHIT and WWOX genes. Briefly, a series of MLPA probes each consisting of 2 target specific hemi probes, one of which is linked to a stuffer sequence of variable length, and with two end sequences recognized by universal MLPA primer pair are hybridized to the test DNA. Once hybridized to the target DNA the 2 hemi probes, designed next to each other, can be ligated and PCR amplified. The stuffer sequence allows for each specific probe set amplification product to have a defined size and to be separated by capillary electrophoresis. For each probe the amount of PCR product obtained reflects the amount of target DNA in the sample and relative target copy number can be measured.
Sixteen probes for the FHIT gene covering all 10 exons (two probes set covering exons 1 to 5 as well as exon 8) and 7 probes for the WWOX gene covering 5 out of 9 exons were developed. Fifteen other probes for other human genes on chromosome 3p and 16q as well as other chromosomes, (8p, 9q, 10p, 12p, 16p), were included as internal control.
The MLPA reaction was performed as previously described  using ± 200 ng of DNA from each cell line and primary tumor.
Experimental data analysis
The PCR amplified products were separated by capillary electrophoresis on an ABI 3100 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). The peak heights values were obtained by Gene Scan analysis software (Applied Biosystems) and exported to an excel spreadsheet for further processing.
Ten DNA samples extracted from healthy individuals were first analyzed to assess the pattern of probe amplification in normal tissue, to create a mean "reference control" pattern, and to exclude the possibility of copy number polymorphism. For each probe in each sample run, the relative peak height was calculated by dividing its absolute value by the sum of all probes peak height for this run. A mean normalized peak height was calculated for each probe and standard deviation established. All five ESCC tumor cell lines were then processed in duplicate from cell cultures grown at different time and with differing DNA extractions. External control samples were included in each run. The cell lines amplification patterns were consistent across runs. The ratio of each cell line probe peaks to the control sample probe peaks was first examined using relative peak height values.
Since a large number of chromosomal abnormalities may be present in cancer cells, the normalization of probe peaks by relative values (percentage) can distort the overall profile of probe amplification. Normalization to the ratio of the absolute peak height of 'control probes' in the tumor by the peak height of 'control probes' in the external reference DNA is therefore preferable and has been used with differing MLPA probes set [37, 38]. Assigning this ratio a value of one, it can be applied to normalize all peaks in a run. The choice of adequate control probes is important due to the number of genetic alterations that may be present in cancer cells and affect "control probes" themselves. Here we have used an absolute peak ratio, selecting internal control probes whose pattern of amplification did not deviate from that of the reference control in the same run as seen on the original Gene Scan data.
Amplification pattern in controls
Comparison of FHIT and WWOXDNA copy number in cell lines by MLPA and FISH
Summary of FISH, MLPA and RT-PCR results for FHIT on the five ESCC cell lines.
Modal chromosome number
FISH 3p copy number*
MLPA FHITexon dosage deletion
Homozygous deletion exon 5
Aberrant transcripts only
2+ 2 small
Hemizygous deletion exons: 1 to 10
Normal transcript and weak expression
Hemizygous deletion exons 4 and 5
Aberrant transcript only and weak expression
Hemizygous deletions exons 1, 3, 4, 5, 7, 10. (preferential 4 and 5)
Aberrant transcript only and weak expression
By contrast in none of the cell lines WWOX deletions could be detected, with all ratios having values above 1 (but below 1.3). Cell line WHCO6 showed an increased copy number for exon 1 and 9; chromosome 16 q was however present in 3 to 4 copies in this cell line shown by FISH analysis with a chromosome 16q library, (results not shown). This was paralleled by an increased MLPA signal for some of the control probes mapping to 16q and included in this assay.
RT-PCR on WWOX showed a normal amplification product size in all cell lines (results not shown). The combined information of MLPA, FISH and RT-PCR is summarized in Table 1.
Evaluation of FHIT and WWOXDNA copy number in primary tumors by MLPA
This study describes the value of an innovative approach using an MLPA assay specifically designed to scan the exon intragenic composition of the FHIT and WWOX genes involved at fragile sites, here evaluated in the setting of ESCC South African cell lines and primary tumor specimens.
Many studies have used LOH, assays to detect large intragenic deletion in genes at fragile sites [40–42]. However, deletions occurring within fragile site are themselves heterogeneous [1, 43], they can be discontinuous and do not always affect coding exons . In this approach, the detection of deletions is reflected by ratios (Figure 3) and does not depend on the presence of informative markers. Hemi and homozygous genomic deletions encompassing FHIT and WWOX exons were targeted to reflect the exon dosage composition of the genes. The internal control probe used to normalize the peak heights should be selected in each cancer sample and should have an amplification pattern consistent with its equivalent in control samples included in the same run. Fifteen internal control probes dispersed in the genome expanded the possibilities.
In this study there was a correlation between the presence of FHIT exon deletions and aberrant pattern of RNA expression in the cell lines. FHIT expression was generally very low in the established ESCC cell lines. Due to its exon based design, this assay would miss intronic genomic deletions that do not affect exon integrity but may inhibit FHIT expression as was observed in some studies . In this regard it is worth noting that since most of the FHIT/WWOX probes map within exons, the assay could be used for RNA studies.
Despite some controversy regarding the involvement of FHIT in oncogenesis there is mounting evidence to suggest that it is more than a passive actor, although the nature of its role in differing cancer scenarios subtypes remains to be elucidated. FHIT is altered in a wide variety of tumors, mostly by genomic deletions and/or promoter hypermethylation, which results in inhibition of the FHIT product. Several reports have raised pertinent questions regarding the relevance of FHIT deletion to cancer development: exon skipping alternative transcripts have been found in normal tissues in addition to normal transcripts [44–46], the gene lies within an instable genomic region which may be mechanistically deleted and the effect of hemizygous deletion in tumors is unclear. Experimental models have shown that FHIT full or haplo insufficiency confers an increased sensitivity to carcinogen exposure . FHIT has an anti-apoptotic activity  and it is possible that FHIT decreased expression facilitates the occurrence of added deleterious genetic events.
Four of five SA ESCC cell lines and four of ten primary tumors had a FHIT deletion suggesting that FHIT might be of relevance in SA esophageal cancer as found in other parts of the world where the incidence of this type of cancer is high [7, 31]. Exons 4 and 5 tended to be the preferential target in three cell lines and two primary tumors, as in previous observations [1, 49]. Cell line SNO had a homozygous deletion of exon 5 and no functional transcript. The other three cell lines had hemizygous deletion of FHIT exons, with an abnormal expression pattern. The evaluation of chromosome 3p copy number by FISH as well as the internal control probes on 3p in MLPA established that these deletions were specific for the FRA3B region in ESCC cell lines and in three of four primary tumors. Two of the primary tumors, had an MPLA profile consistent with hemizygous deletion of the FHIT locus. FHIT has previously been investigated in South African oral squamous cell carcinoma (OSCC) [50, 51] which is known to share similar etiological risk factors with ESCC. In one study, the authors found reduced or absent Fhit protein in 12 of 17 tumors and aberrant RT-PCR products in a third of the cases . In the present study, FHIT deletions were associated with aberrant transcripts in the cell lines and deletions were observed in a third of primary ESCC tumors. It would be necessary to screen a larger cohort of primary tumors to evaluate the importance of FHIT and WWOX intragenic deletions in SA ESCC.
In contrast to FHIT, no deletion was observed in WWOX in either the cell lines or the primary tumors. Some studies have shown that the simultaneous loss of expression of FHIT and WWOX is frequent in several cancers [52–54]. Since all common fragile sites are sensitive to external carcinogens these findings raise questions as to the exact mechanism involved in these cell lines. FHIT loci could have been altered early in the disease process and a clone be driven/facilitated by its loss with FRA 3B being the most sensitive fragile site . Deletions could also represent a later event where FHIT haplo-insufficiency aggravated an already unstable genetic background. WWOX might be concordantly inactivated via a differing mechanism. While LOH at the WWOX locus has been reported in ESCC , WWOX is frequently inactivated by promoter hypermethylation [57, 58], which was not detected in this assay.
FHIT loss, with or without WWOX loss, has been shown to correlate positively with tumor invasiveness and prognosis in several cancers (breast, pancreas, gastric cancers) [54, 59, 60]. At the same time, Fhit protein loss can be detected early in some cancer, including ESCC, and appears to correlate with the stages of malignant transformation . Whether involved early in the disease or deleted as a consequence of genomic instability and enrolled as a partner in the progression of the malignant phenotype it will be important to establish the role of genes at fragile sites in relation to specific carcinogen exposure and disease behavior. Interestingly two recent and independents studies have shown that both in patients and experimentally induced pre-malignant lesions there is an activation of the DNA damage response and increased genetic instability at fragile sites, before genomic instability [61, 62]. The detection of genetic imbalances at fragile sites may therefore be of value to detect the "signature" of replication stress  and may assist to characterize pre-malignant lesions.
The FHIT/WWOX assay described in this study may offer a rapid and cost effective method to assist a deletion screening of tissue lesions associated with heavy carcinogen exposure, (esophageal, head and neck, and lung pathologies). It may help clarify whether genes at fragile sites tend to be concordantly deleted.
In this study, the assay detected FHIT deletions in four of five SA ESCC cell lines and four of ten primary tumors, which justifies a wider screening in SA ESCC patients. The FHIT/WWOX MLPA kit is likely to be a valuable tool in other cancer where its prognostic and early screening value will have to be assessed further.
This work was supported by the Cancer Association of South Africa CANSA, and by the National Health Laboratory Services, NHLS research trust. The probes were developed and provided by MRC Holland.
We thank Rob Veale for providing the five-esophageal carcinoma cell lines, R Ally and H Hassan for the collection of primary tumors, Wayne Grayson for histological assessment of biopsies, Wendy Stevens, and Patrick Arbuthnot for critically reading this paper.
- Corbin S, Neilly ME, Espinosa R, Davis EM, McKeithan TW, Le Beau MM: Identification of unstable sequences within the common fragile site at 3p14.2: implications for the mechanism of deletions within fragile histidine triad gene/common fragile site at 3p14.2 in tumors. Cancer Res. 2002, 62 (12): 3477-3484.PubMed
- Bednarek AK, Laflin KJ, Daniel RL, Liao Q, Hawkins KA, Aldaz CM: WWOX, a novel WW domain-containing protein mapping to human chromosome 16q23.3-24.1, a region frequently affected in breast cancer. Cancer Res. 2000, 60 (8): 2140-2145.PubMed
- Ohta M, Inoue H, Cotticelli MG, Kastury K, Baffa R, Palazzo J, Siprashvili Z, Mori M, McCue P, Druck T, Croce CM, Huebner K: The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell. 1996, 84 (4): 587-597. 10.1016/S0092-8674(00)81034-X.View ArticlePubMed
- Matsuyama A, Croce CM, Huebner K: Common fragile genes. Eur J Histochem. 2004, 48 (1): 29-36.PubMed
- Huebner K, Croce CM: FRA3B and other common fragile sites: the weakest links. Nat Rev Cancer. 2001, 1 (3): 214-221. 10.1038/35106058.View ArticlePubMed
- Kuroki T, Trapasso F, Yendamuri S, Matsuyama A, Alder H, Mori M, Croce CM: Allele loss and promoter hypermethylation of VHL, RAR-beta, RASSF1A, and FHIT tumor suppressor genes on chromosome 3p in esophageal squamous cell carcinoma. Cancer Res. 2003, 63 (13): 3724-3728.PubMed
- Kitamura A, Yashima K, Okamoto E, Andachi H, Hosoda A, Kishimoto Y, Shiota G, Ito H, Kaibara N, Kawasaki H: Reduced Fhit expression occurs in the early stage of esophageal tumorigenesis: no correlation with p53 expression and apoptosis. Oncology. 2001, 61 (3): 205-211. 10.1159/000055376.View ArticlePubMed
- Mori M, Mimori K, Shiraishi T, Alder H, Inoue H, Tanaka Y, Sugimachi K, Huebner K, Croce CM: Altered expression of Fhit in carcinoma and precarcinomatous lesions of the esophagus. Cancer Res. 2000, 60 (5): 1177-1182.PubMed
- Noguchi T, Takeno S, Kimura Y, Uchida Y, Daa T, Yokoyama S, Gabbert HE, Mueller W: FHIT expression and hypermethylation in esophageal squamous cell carcinoma. Int J Mol Med. 2003, 11 (4): 441-447.PubMed
- National Cancer Registry of South Africa: . 2004, Johannesburg , National Cancer Registry of South Africa, National Health Laboratory
- Pacella-Norman R, Urban MI, Sitas F, Carrara H, Sur R, Hale M, Ruff P, Patel M, Newton R, Bull D, Beral V: Risk factors for oesophageal, lung, oral and laryngeal cancers in black South Africans. Br J Cancer. 2002, 86 (11): 1751-1756. 10.1038/sj.bjc.6600338.PubMed CentralView ArticlePubMed
- Marasas WF, van Rensburg SJ, Mirocha CJ: Incidence of Fusarium species and the mycotoxins, deoxynivalenol and zearalenone, in corn produced in esophageal cancer areas in Transkei. J Agric Food Chem. 1979, 27 (5): 1108-1112. 10.1021/jf60225a013.View ArticlePubMed
- Dandara C, Ballo R, Parker MI: CYP3A5 genotypes and risk of oesophageal cancer in two South African populations. Cancer Lett. 2005, 225 (2): 275-282. 10.1016/j.canlet.2004.11.004.View ArticlePubMed
- Isaacson C: The change of the staple diet of black South Africans from sorghum to maize (corn) is the cause of the epidemic of squamous carcinoma of the oesophagus. Med Hypotheses. 2005, 64 (3): 658-660. 10.1016/j.mehy.2004.09.019.View ArticlePubMed
- Matsha T, Erasmus R, Kafuko AB, Mugwanya D, Stepien A, Parker MI: Human papillomavirus associated with oesophageal cancer. J Clin Pathol. 2002, 55 (8): 587-590. 10.1136/jcp.55.8.587.PubMed CentralView ArticlePubMed
- Stein CK, Glover TW, Palmer JL, Glisson BS: Direct correlation between FRA3B expression and cigarette smoking. Genes Chromosomes Cancer. 2002, 34 (3): 333-340. 10.1002/gcc.10061.View ArticlePubMed
- Thorland EC, Myers SL, Gostout BS, Smith DI: Common fragile sites are preferential targets for HPV16 integrations in cervical tumors. Oncogene. 2003, 22 (8): 1225-1237. 10.1038/sj.onc.1206170.View ArticlePubMed
- Stevens VL, Tang J: Fumonisin B1-induced sphingolipid depletion inhibits vitamin uptake via the glycosylphosphatidylinositol-anchored folate receptor. J Biol Chem. 1997, 272 (29): 18020-18025. 10.1074/jbc.272.29.18020.View ArticlePubMed
- Capaccio P, Ottaviani F, Cuccarini V, Cenzuales S, Cesana BM, Pignataro L: Association between methylenetetrahydrofolate reductase polymorphisms, alcohol intake and oropharyngolaryngeal carcinoma in northern Italy. J Laryngol Otol. 2005, 119 (5): 371-376.PubMed
- Yokoyama T, Saito K, Lwin H, Yoshiike N, Yamamoto A, Matsushita Y, Date C, Tanaka H: Epidemiological evidence that acetaldehyde plays a significant role in the development of decreased serum folate concentration and elevated mean corpuscular volume in alcohol drinkers. Alcohol Clin Exp Res. 2005, 29 (4): 622-630. 10.1097/01.ALC.0000158842.24218.03.View ArticlePubMed
- Knasmuller S, Bresgen N, Kassie F, Mersch-Sundermann V, Gelderblom W, Zohrer E, Eckl PM: Genotoxic effects of three Fusarium mycotoxins, fumonisin B1, moniliformin and vomitoxin in bacteria and in primary cultures of rat hepatocytes. Mutat Res. 1997, 391 (1-2): 39-48.View ArticlePubMed
- Lerda D, Biaggi Bistoni M, Peralta N, Ychari S, Vazquez M, Bosio G: Fumonisins in foods from Cordoba (Argentina), presence and genotoxicity. Food Chem Toxicol. 2005, 43 (5): 691-698. 10.1016/j.fct.2004.12.019.View ArticlePubMed
- Xiao YP, Wu DY, Xu L, Xin Y: Loss of heterozygosity and microsatellite instabilities of fragile histidine triad gene in gastric carcinoma. World J Gastroenterol. 2006, 12 (23): 3766-3769.PubMed CentralPubMed
- Liu H, Peng ZL, Wang H, Liu SL, Zhang CS, Tang QP, He B: [Study on the relationship between LOH and MI of FHIT gene and the development of cervical carcinoma]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2005, 36 (4): 506-509.PubMed
- Boldog F, Gemmill RM, West J, Robinson M, Robinson L, Li E, Roche J, Todd S, Waggoner B, Lundstrom R, Jacobson J, Mullokandov MR, Klinger H, Drabkin HA: Chromosome 3p14 homozygous deletions and sequence analysis of FRA3B. Hum Mol Genet. 1997, 6 (2): 193-203. 10.1093/hmg/6.2.193.View ArticlePubMed
- Chen PM, Yang MH, Hsiao LT, Yu IT, Chu CJ, Chao TC, Yen CC, Wang WS, Chiou TJ, Liu JH: Decreased FHIT protein expression correlates with a worse prognosis in patients with diffuse large B-cell lymphoma. Oncol Rep. 2004, 11 (2): 349-356.PubMed
- Guler G, Uner A, Guler N, Han SY, Iliopoulos D, Hauck WW, McCue P, Huebner K: The fragile genes FHIT and WWOX are inactivated coordinately in invasive breast carcinoma. Cancer. 2004, 100 (8): 1605-1614. 10.1002/cncr.20137.View ArticlePubMed
- Guerin LA, Hoffman HT, Zimmerman MB, Robinson RA: Decreased fragile histidine triad gene protein expression is associated with worse prognosis in oral squamous carcinoma. Arch Pathol Lab Med. 2006, 130 (2): 158-164.PubMed
- Sarli L, Bottarelli L, Azzoni C, Campanini N, Di Cola G, Bader G, Iusco D, Salvemini C, Caruso G, Donadei E, Pizzi S, D'Adda T, Renato C, Roncoroni L, Bordi C: Abnormal Fhit protein expression and high frequency of microsatellite instability in sporadic colorectal cancer. Eur J Cancer. 2004, 40 (10): 1581-1588. 10.1016/j.ejca.2004.02.021.View ArticlePubMed
- Iliopoulos D, Guler G, Han SY, Johnston D, Druck T, McCorkell KA, Palazzo J, McCue PA, Baffa R, Huebner K: Fragile genes as biomarkers: epigenetic control of WWOX and FHIT in lung, breast and bladder cancer. Oncogene. 2005, 24 (9): 1625-1633. 10.1038/sj.onc.1208398.View ArticlePubMed
- Liu FX, Huang XP, Zhao CX, Xu X, Han YL, Cai Y, Wu RL, Wu M, Zhan QM, Wang MR: [Allelic loss and down-regulation of FHIT gene expression in esophageal squamous cell carcinoma]. Ai Zheng. 2004, 23 (9): 992-998.PubMed
- Druck T, Hadaczek P, Fu TB, Ohta M, Siprashvili Z, Baffa R, Negrini M, Kastury K, Veronese ML, Rosen D, Rothstein J, McCue P, Cotticelli MG, Inoue H, Croce CM, Huebner K: Structure and expression of the human FHIT gene in normal and tumor cells. Cancer Res. 1997, 57 (3): 504-512.PubMed
- Schouten JP, McElgunn CJ, Waaijer R, Zwijnenburg D, Diepvens F, Pals G: Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification. Nucleic Acids Res. 2002, 30 (12): e57-10.1093/nar/gnf056.PubMed CentralView ArticlePubMed
- Bey E, Alexander J, Whitcutt JM, Hunt JA, Gear JH: Carcinoma of the esophagus in Africans: establishment of a continuously growing cell line from a tumor specimen. In Vitro. 1976, 12 (2): 107-114.View ArticlePubMed
- Veale RB, Thornley A: Increased single class low-affinity EGF receptors expressed by human oesophageal squamous cell carcinoma lines. South African Journal of Science. 1989, South Africa , 85: 375-379.
- Fang JM, Arlt MF, Burgess AC, Dagenais SL, Beer DG, Glover TW: Translocation breakpoints in FHIT and FRA3B in both homologs of chromosome 3 in an esophageal adenocarcinoma. Genes Chromosomes Cancer. 2001, 30 (3): 292-298. 10.1002/1098-2264(2000)9999:9999<::AID-GCC1095>3.0.CO;2-F.View ArticlePubMed
- Buffart TE, Coffa J, Hermsen MA, Carvalho B, van der Sijp JR, Ylstra B, Pals G, Schouten JP, Meijer GA: DNA copy number changes at 8q11-24 in metastasized colorectal cancer. Cell Oncol. 2005, 27 (1): 57-65.PubMed CentralPubMed
- Postma C, Hermsen MA, Coffa J, Baak JP, Mueller JD, Mueller E, Bethke B, Schouten JP, Stolte M, Meijer GA: Chromosomal instability in flat adenomas and carcinomas of the colon. J Pathol. 2005, 205 (4): 514-521. 10.1002/path.1733.View ArticlePubMed
- Vorstman JA, Jalali GR, Rappaport EF, Hacker AM, Scott C, Emanuel BS: MLPA: a rapid, reliable, and sensitive method for detection and analysis of abnormalities of 22q. Hum Mutat. 2006
- Lee YC, Wu CT, Shih JY, Jou YS, Chang YL: Frequent allelic deletion at the FHIT locus associated with p53 overexpression in squamous cell carcinoma subtype of Taiwanese non-small-cell lung cancers. Br J Cancer. 2004, 90 (12): 2378-2383.PubMed CentralPubMed
- Cheung AL, Si HX, Wang LD, An JY, Tsao SW: Loss of heterozygosity analyses of esophageal squamous cell carcinoma and precursor lesions from a high incidence area in China. Cancer Lett. 2005
- Santos SC, Cavalli LR, Cavalli IJ, Lima RS, Haddad BR, Ribeiro EM: Loss of heterozygosity of the BRCA1 and FHIT genes in patients with sporadic breast cancer from Southern Brazil. J Clin Pathol. 2004, 57 (4): 374-377. 10.1136/jcp.2003.013490.PubMed CentralView ArticlePubMed
- Mimori K, Druck T, Inoue H, Alder H, Berk L, Mori M, Huebner K, Croce CM: Cancer-specific chromosome alterations in the constitutive fragile region FRA3B. Proc Natl Acad Sci U S A. 1999, 96 (13): 7456-7461. 10.1073/pnas.96.13.7456.PubMed CentralView ArticlePubMed
- Chen YJ, Chen PH, Lee MD, Chang JG: Aberrant FHIT transcripts in cancerous and corresponding non-cancerous lesions of the digestive tract. Int J Cancer. 1997, 72 (6): 955-958. 10.1002/(SICI)1097-0215(19970917)72:6<955::AID-IJC6>3.0.CO;2-O.View ArticlePubMed
- Chu TY, Shen CY, Chiou YS, Lu JJ, Perng CL, Yu MS, Liu HS: HPV-associated cervical cancers show frequent allelic loss at 3p14 but no apparent aberration of FHIT mRNA. Int J Cancer. 1998, 75 (2): 199-204. 10.1002/(SICI)1097-0215(19980119)75:2<199::AID-IJC6>3.0.CO;2-P.View ArticlePubMed
- Matthews CP, Shera K, Kiviat N, McDougall JK: Expression of truncated FHIT transcripts in cervical cancers and in normal human cells. Oncogene. 2001, 20 (34): 4665-4675. 10.1038/sj.onc.1204622.View ArticlePubMed
- Zanesi N, Fidanza V, Fong LY, Mancini R, Druck T, Valtieri M, Rudiger T, McCue PA, Croce CM, Huebner K: The tumor spectrum in FHIT-deficient mice. Proc Natl Acad Sci U S A. 2001, 98 (18): 10250-10255. 10.1073/pnas.191345898.PubMed CentralView ArticlePubMed
- Sard L, Accornero P, Tornielli S, Delia D, Bunone G, Campiglio M, Colombo MP, Gramegna M, Croce CM, Pierotti MA, Sozzi G: The tumor-suppressor gene FHIT is involved in the regulation of apoptosis and in cell cycle control. Proc Natl Acad Sci U S A. 1999, 96 (15): 8489-8492. 10.1073/pnas.96.15.8489.PubMed CentralView ArticlePubMed
- Tanimoto K, Hayashi S, Tsuchiya E, Tokuchi Y, Kobayashi Y, Yoshiga K, Okui T, Kobayashi M, Ichikawa T: Abnormalities of the FHIT gene in human oral carcinogenesis. Br J Cancer. 2000, 82 (4): 838-843. 10.1054/bjoc.1999.1009.PubMed CentralView ArticlePubMed
- van Heerden WF, Swart TJ, Robson B, Smith TL, Engelbrecht S, van Heerden MB, van Rensburg EJ, Huebner K: FHIT RNA and protein expression in oral squamous cell carcinomas. Anticancer Res. 2001, 21 (4A): 2425-2428.PubMed
- van Heerden WF, Swart TJ, van Heerden MB, Pekarsky Y, Sutherland R, Huebner K: Fhit protein expression in oral epithelium: immunohistochemical evaluation of three antisera. Anticancer Res. 2001, 21 (4A): 2419-2423.PubMed
- Ishii H, Vecchione A, Furukawa Y, Sutheesophon K, Han SY, Druck T, Kuroki T, Trapasso F, Nishimura M, Saito Y, Ozawa K, Croce CM, Huebner K, Furukawa Y: Expression of FRA16D/WWOX and FRA3B/FHIT genes in hematopoietic malignancies. Mol Cancer Res. 2003, 1 (13): 940-947.PubMed
- Ishii H, Furukawa Y: Alterations of common chromosome fragile sites in hematopoietic malignancies. Int J Hematol. 2004, 79 (3): 238-242.View ArticlePubMed
- Guler G, Uner A, Guler N, Han SY, Iliopoulos D, McCue P, Huebner K: Concordant loss of fragile gene expression early in breast cancer development. Pathol Int. 2005, 55 (8): 471-478. 10.1111/j.1440-1827.2005.01855.x.View ArticlePubMed
- Glover TW, Stein CK: Induction of sister chromatid exchanges at common fragile sites. Am J Hum Genet. 1987, 41 (5): 882-890.PubMed CentralPubMed
- Kuroki T, Trapasso F, Shiraishi T, Alder H, Mimori K, Mori M, Croce CM: Genetic alterations of the tumor suppressor gene WWOX in esophageal squamous cell carcinoma. Cancer Res. 2002, 62 (8): 2258-2260.PubMed
- Kuroki T, Yendamuri S, Trapasso F, Matsuyama A, Aqeilan RI, Alder H, Rattan S, Cesari R, Nolli ML, Williams NN, Mori M, Kanematsu T, Croce CM: The tumor suppressor gene WWOX at FRA16D is involved in pancreatic carcinogenesis. Clin Cancer Res. 2004, 10 (7): 2459-2465. 10.1158/1078-0432.CCR-03-0096.View ArticlePubMed
- Qin HR, Iliopoulos D, Semba S, Fabbri M, Druck T, Volinia S, Croce CM, Morrison CD, Klein RD, Huebner K: A Role for the WWOX Gene in Prostate Cancer. Cancer Res. 2006, 66 (13): 6477-6481. 10.1158/0008-5472.CAN-06-0956.View ArticlePubMed
- Arun B, Kilic G, Yen C, Foster B, Yardley DA, Gaynor R, Ashfaq R: Loss of FHIT expression in breast cancer is correlated with poor prognostic markers. Cancer Epidemiol Biomarkers Prev. 2005, 14 (7): 1681-1685. 10.1158/1055-9965.EPI-04-0278.View ArticlePubMed
- Aqeilan RI, Kuroki T, Pekarsky Y, Albagha O, Trapasso F, Baffa R, Huebner K, Edmonds P, Croce CM: Loss of WWOX expression in gastric carcinoma. Clin Cancer Res. 2004, 10 (9): 3053-3058. 10.1158/1078-0432.CCR-03-0594.View ArticlePubMed
- Bartkova J, Horejsi Z, Koed K, Kramer A, Tort F, Zieger K, Guldberg P, Sehested M, Nesland JM, Lukas C, Orntoft T, Lukas J, Bartek J: DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature. 2005, 434 (7035): 864-870. 10.1038/nature03482.View ArticlePubMed
- Gorgoulis VG, Vassiliou LV, Karakaidos P, Zacharatos P, Kotsinas A, Liloglou T, Venere M, Ditullio RAJ, Kastrinakis NG, Levy B, Kletsas D, Yoneta A, Herlyn M, Kittas C, Halazonetis TD: Activation of the DNA damage checkpoint and genomic instability in human precancerous lesions. Nature. 2005, 434 (7035): 907-913. 10.1038/nature03485.View ArticlePubMed
- Casper AM, Nghiem P, Arlt MF, Glover TW: ATR regulates fragile site stability. Cell. 2002, 111 (6): 779-789. 10.1016/S0092-8674(02)01113-3.View ArticlePubMed
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/6/205/prepub
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