Expression of methionine adenosyltransferase 2A in renal cell carcinomas and potential mechanism for kidney carcinogenesis
- Xuliang Wang†1, 2,
- Xiaoqiang Guo†1,
- Wenshui Yu2, 3,
- Cailing Li2,
- Yaoting Gui2 and
- Zhiming Cai1, 2Email author
© Wang et al.; licensee BioMed Central Ltd. 2014
Received: 4 November 2013
Accepted: 6 March 2014
Published: 17 March 2014
Methionine adenosyltransferase 2A (MAT2A) is an enzyme that catalyzes the formation of S-adenosylmethionine (SAMe) by joining methionine and ATP. SAMe is a methyl donor for transmethylation and has an important role for DNA and/or protein methylation. MAT2A is expressed widely in many tissues especially in kidney. Several studies have demonstrated that there are abnormal expressions of MAT2A in several kinds of cancers such as liver and colon cancers. But the relationship of MAT2A between renal cell carcinomas (RCC) is less understood.
The mRNA expression level of the MAT2A gene was determined in 24 RCC patients and 4 RCC cell lines, using real-time quantitative-polymerase chain reaction (RT-PCR). The MAT2A protein content was measured by western blotting and immunohistochemical analysis in 55 RCC patients. The mRNA levels of heme oxygenase-1 (HO-1) and cyclooxygenase-2 (COX-2) were also analysized in patients using RT-PCR. The correlations between the MAT2A and HO-1 as well as COX-2 were analyzed with nonparametric Spearman method.
MAT2A transcript was significantly downregulated in cancer tissues compared to normal tissues (P < 0.05). Immunohistochemical analysis and western blotting indicated that level of MAT2A protein was decreased in cancer tissues. The statistical analysis reveals a negative correlation between MAT2A and HO-1 expression in RCC patients and cell lines (P < 0.01).
This study demonstrated that MAT2A was lower expression in cancer tissues, suggesting that it may be involved in the development of RCC. MAT2A is a transcriptional corepressor for HO-1 expression by supplying SAM for methyltransferases, which may be one of potential mechanism of MAT2A as tumor suppressor in kidney carcinogenesis.
KeywordsMethionine adenosyltransferase 2A Renal cell carcinomas S-adenosylmethionine Heme oxygenase-1
Kidney cancer is among the 10 most common cancers, which accounts for 2% to 3% of all adult malignancies and causes 100,000 deaths per year worldwide . The most common hisitologic subtype of kidney cancer is renal cell carcinomas (RCC), of which 70–80% of cases are defined as clear cell renal cell carcinoma (ccRCC) . RCC is generally resistant to chemotherapy and radiation therapy . Radical or partial nephrectomy of the tumor at an early stage remains the mainstay of curative therapy nevertheless up to 40% of the patients relapse after surgery . Unlike other solid malignancies, methods for RCC early diagnosis are lacking but they are critically important because therapeutic efficacy and, hence, survival are tightly linked to the time of diagnosis. Distant metastases are present at the time of initial diagnosis in approximately one third of patients, and the tumor will recur in another third, even after nephrectomy with curative intent . Better understanding of the molecular mechanisms of RCC may hasten identification of new prognostic markers and development of new diagnostic and therapeutic strategies.
Cancer cell metabolism is significantly altered compared with metabolism of normal cells. Significant progresses on genetics of renal cancer have proved that it is a metabolic disease . Several known genes related kidney cancer, such as von Hippel-Lindau (VHL), fumarate hydratase (FH) and succinate dehydrogenase (SDH) are involved in pathways that respond to metabolic stress . VHL loss can increase the expression of hypoxia-inducible factors, which affect several metabolic pathways, including glycolysis and oxidative phosphorylation . The mutations of FH and SDH are associated with dysfunction of tricarboxylic acid cycle [9, 10]. So, it will provide the foundation for the development of effective therapy for kidney cancer to understand the metabolic basis of this disease .
One-carbon metabolism can integrate nutritional status from amino acids, glucose and vitamins, which is important for the biosynthesis of lipids, nucleotides and proteins, the maintenance of redox status and the substrates for methylation reactions . One-carbon metabolism involves in the folate and methionine cycles. The related enzymes involved folate metabolism have been discovered to be associated to RCC risk [13, 14]. The abnormality of methionine cycle was identified in many kinds of cancers [15, 16]. But, the relationship between methionine metabolism and RCC is poorly understood. Methionine adenosyltransferase (MAT) is an essential cellular enzyme that catalyzes the formation of S-adenosylmethionine (SAMe), the principal biological methyl donor . In mammals, this essential enzyme is the product of two different genes, MAT1A and MAT2A, which display a distinct pattern of expression among different tissues. MAT1A is the predominant enzyme in liver parenchymal cells, while MAT2A is expressed in all other tissues .
However, the relationship between the expression of MAT2A and RCC development is still unknown. In this study, we investigated expression levels of MAT2A gene and protein in RCC specimen and cell lines. Then, we also determined the association between MAT2A expression and other RCC related genes’ expressions to understand the potential mechanism underlying MAT2A involved in RCC carcinogenesis. Our results suggest that MAT2A is downregulated in cancer tissues of RCC patients and has function of tumor suppressor though repressing the expression of heme oxygenase-1 (HO-1).
Patients and tissue specimens
Summary of the clinical characteristics of 55 RCC patients
Number of patients
All resection samples were confirmed to be ccRCC by clinical pathology and carbonic anhydrase 9 (CA-9) measurements (Additional file 1: Figure S1). The collection and use of the patient samples were reviewed and approved by Institutional Ethics Committees of Peking University Shenzhen Hospital, and written informed consent from all patients was appropriately obtained. Frozen tissues from 24 ccRCC cancer and adjacent normal samples were randomly selected from all 55 paired samples for extraction of total RNA.
The human renal cancer cell lines (ACHN, Caki-1,769-P and 786-O) and embryonic kidney cell (HEK293) were obtained from cell resource center of Shanghai Institutes for Biological Sciences, Chinese Academy of Science. All cells were maintained in Dulbecco’s modified Eagle’s medium (GIBCO, Grand Island, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, Logan, USA), 50 U/mL penicillin and 50 μg/mL streptomycin. Cells were grown in a humidified atmosphere with 5% CO2 at 37°C. Cells were collected for following study.
RNA extraction and cDNA synthesis
Total RNA was extracted from cancer tissues, normal adjacent tissues and 5 cells with Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. The concentration of total RNA was determined using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). Then, cDNA was synthesized from 1 μg of total RNA using a Fermentas RT system (Thermo Scientific, Wilmington, DE, USA), according to the manufacturer’s instructions. Reverse transcription reactions were carried out at 25°C for 5 mins and followed by 42°C for 60 mins.
Quantitative real-time polymerase chain reaction (qRT-PCR)
The mRNA expression levels were analyzed using SYBR Premix Ex TaqTM II (Takara, Dalian, China), with β-Actin as an internal reference. qRT-PCR was performed in 20 μl reaction mixture containing 10 μl of SYBR Premix, 0.5 μM of forward and reverse primers, and 1 μl template cDNA on LightCycler480 System (Roche, Foster City, CA, USA). The primers were designed according to the human MAT2A, CA-9, heme oxygenase-1(HO-1), cyclooxygenase-2 (COX-2) and β-Actin genes sequences reported in GenBank. The primer sequences were synthesized by Invitrogen (Guangzhou, China) as follows:
MAT2A, Forward primer: 5′-ATGAACGGACAGCTCAACGG-3′,
Reverse primer: 5′-CCAGCAAGAAGGATCATTCCAG-3′;
CA-9, Forward primer: 5′- GGATCTACCTACTGTTGAGGCT-3′,
Reverse primer: 5′- CATAGCGCCAATGACTCTGGT-3′;
HO-1, Forward primer: 5′-ATGACACCAAGGACCAGAGC -3′,
Reverse primer: 5′-GTGTAAGGACCCATCGGAGA -3′;
COX-2, Forward primer: 5′-CTGGCGCTCAGCCATACAG-3′,
Reverse primer: 5′-CGCACTTATACTGGTCAAATCCC-3′;
β-Actin, Forward primer: 5′- CCACTGGCATCGTGATGGACTCC -3′,
Reverse primer: 5′-GCCGTGGTGGTGAAGCTGTAGC-3′;
All reactions were incubated at 95°C for 5 min, followed by 40 cycles of 95°C for 10 s, 60°C for 20 s and 72°C for 30 s. PCR reactions of each sample were conducted in duplicate. Data were analyzed through the comparative threshold cycle (CT) method.
Five cells, cancer tissues and adjacent normal tissues from all patients were homogenized in radioimmunoprecipitation assay buffer (RIPA) containing the protease inhibitors phenylmethylsulfonyl fluoride (100 μg/mL), cocktail (1 mmol/L) and dithiothreitol (0.5 mmol/L). Homogenates were centrifuged and supernatants were collected. Protein concentrations were determined by bicinchoninic acid (BCA) protein assay kit (Thermo Pierce ). A total of 50 μg of protein from each sample was resolved by reducing loading buffer and separated by 10% sodiumdodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by electrophoretic transfer to a polyvinylidene difluoride (PVDF) membrane. The PVDF membrane was saturated with 5% skim milk in TBST (50 mM Tris–HCl, 150 mM NaCl, 0.1% Tween-20) for 2 h and then incubated with primary antibodies at 4°C overnight. The primary antibodies used included rabbit polyclonal antibodies to MAT2A (1:1000, Abcam, Hong Kong, China), HO-1(1:200, Santa Cruz, Shanghai, China) and β-actin (1:5,000, Abcam, Hong Kong, China). The specificity of the MAT2A antibody has been determined (Additional file 2: Figure S2). PVDF membrane was incubated with 1:10,000-diluted peroxidase-coupled goat anti-rabbit immunoglobulin G (IgG) (secondary antibody, EarthOx, San Francisco, USA) for 1 h, after washing three times with TBST (5 min/time) at room temperature. After further washing with TBST four times, the PVDF membrane was exposed to enhanced chemiluminescence substrate (Millipore, Rockford, USA) for 30 min and detection was performed using a film.
Paraffin sections (3 μm) from samples of 55 ccRCC samples and adjacent normal samples were deparaffinized in 100% xylene and re-hydrated in descending ethanol series (100%, 90%, 80%, 70% ethanol) and water according to standard protocol. Heat-induced antigen retrieval was performed in 10 mM citrate buffer for 2 min at 100°C. Endogenous peroxidase activity and non-specific antigen were blocked with peroxidase blocking reagent containing 3% hydrogen peroxide and serum, followed by incubation with rabbit anti-human MAT2A antibody for 1 h at 37°C. After washing, the sections were incubated with biotin-labelled goat anti-rabbit antibody for 10 min at room temperature, and subsequently were incubated with streptavidin-conjugated horseradish peroxidase (HRP) (Maixin Inc, China). The peroxidase reaction was developed using 3, 3-diaminobenzidine chromogen solution in DAB buffer substrate. Sections were visualized with DAB and counterstained with hematoxylin, mounted in neutral gum, and analyzed using a bright field microscope.
All of the IHC staining results were reviewed independently by two pathologists. Positive expression of MAT2A was defined as the brown staining in the cytoplasm and nucleus. The staining results for MAT2A were semiquantitatively scored. Intensity was estimated in comparison to the control and scored as follows: 0, negative staining; 1, weak staining; 2, moderate staining; and 3, strong staining. Scores representing the percentage of tumor cells stained positive were as follows: 0, no positive cell; 1, <5%; 2, 6–25%; 3, 26–50%; 4, 51–75%; and 5, > 75%. A final score was calculated by multiplying the scores for intensity and percentage.
Statistical analysis was carried out using the SPSS 13.0 statistical software package. qRT-PCR and immunohistochemical data were analyzed by two-tailed paired t-test and Mann–Whitney U test (α = 0.05). The nonparametric Spearman rank correlation coefficient was used to calculate the correlation between the MAT2A and HO-1 as well as COX-2 expressions. For all analyses, p < 0.05 was considered significant.
Downregulated mRNA expression of MAT2A in ccRCC patients and kidney cancer cell lines
Reduced protein content of MAT2A in ccRCC
Negative correlation of gene expression between MAT2A and HO-1
Both DNA and histone methylation are important regulators for gene expression and chromatin structure, which have multiple effects on carcinogenesis [19, 20], but the detailed mechanism is required to be determined. As a methyl donor, SAMe also plays vital role in gene expression via its effect on methylation . So, MAT2A has a potential effect on tumor development and progression . Recent studies have illustrated there are abnormal expressions of MAT2A in some tumors, including liver, gastric and colon cancers [23–25]. In our study, the content of MAT2A is obviously decreased in cancer tissue of RCC patients under mRNA and protein levels. So, MAT2A functions as a tumor suppressor in RCC. An increasing number of studies have suggested that MAT2A plays an important pathogenetic role in facilitating liver and colon cancer growth [26, 27]. Our results further provide evidence that abnormal MAT2A is also a factor of RCC development.
Previous studies have indicated HO-1 and COX-2 are regulated by MAT2A . HO-1 is an enzyme that catalyzes the degradation of heme and affords protection against programmed cell death. HO-1 is vital to fumarate hydratase deficient kidney cells survival and inhibition of it can lead to cell death . It has been demonstrated HO-1 is often overexpressed in RCC patients and cell lines, and promotes survival of renal cancer cells [30, 31]. COX-2 is an enzyme which catalyzes the synthesis of prostaglandins from arachidonic acid. It has been also demonstrated that COX-2 is increased in RCC and plays an important role in the proliferation of malignant renal cells [32, 33]. Our results also confirmed both HO-1 and COX-2 are upregulated in RCC patients and cell lines, but further evidence indicates MAT2A is negative correlation with HO-1, no COX-2. It means that MAT2A biological role in RCC seems to be mainly associated with HO-1.
In summary, our results reveal that downregulated expression level of MAT2A is common in cancer tissues of RCC patients. The reduced MAT2A may derepress the expression of HO-1 through lowering DNA and/or histone methylation, which can be considered as potential cause of MAT2A involved RCC suppression. The results also imply that identification of other genes regulated by MAT2A during RCC development will expand our understanding of the carcinogenesis and screening strategies in RCC. Because samples in our study are limited, whether MAT2A can be as biomarker for the early diagnosis of RCC and prognostic evaluation is to be further determined. Our study only provides a possible mechanism of MAT2A biological role, so additional research is also required to determine the link between lower MAT2A levels and RCC development.
This research was supported by Emerging Scientist Project of Shenzhen Second People’s Hospital (No 2012001), the promotion Program for Shenzhen Key Laboratory (ZDSY20120615154448514) and National Natural Science Foundation of China (No 81270740).
- Rini BI, Campbell SC, Escudier B: Renal cell carcinoma. Lancet. 2009, 373 (9669): 1119-1132. 10.1016/S0140-6736(09)60229-4.View ArticlePubMedGoogle Scholar
- Lopez-Beltran A, Carrasco JC, Cheng L, Scarpelli M, Kirkali Z, Montironi R: 2009 update on the classification of renal epithelial tumors in adults. Int J Urol. 2009, 16 (5): 432-443. 10.1111/j.1442-2042.2009.02302.x.View ArticlePubMedGoogle Scholar
- Basu B, Eisen T: Perspectives in drug development for metastatic renal cell cancer. Target Oncol. 2010, 5 (2): 139-156. 10.1007/s11523-010-0149-2.View ArticlePubMedGoogle Scholar
- Ficarra V, Schips L, Guille F, Li G, De La Taille A, Prayer Galetti T, Cindolo L, Novara G, Zigeuner RE, Bratti E, Tostain J, Altieri V, Abbou CC, Artibani W, Patard JJ: Multiinstitutional European validation of the 2002 TNM staging system in conventional and papillary localized renal cell carcinoma. Cancer. 2005, 104 (5): 968-974. 10.1002/cncr.21254.View ArticlePubMedGoogle Scholar
- Mohammed A, Shergill I, Little B: Management of metastatic renal cell carcinoma: current trends. Expert Rev Mol Diagn. 2009, 9 (1): 75-83. 10.1586/1473722.214.171.124.View ArticlePubMedGoogle Scholar
- Linehan WM, Srinivasan R, Schmidt LS: The genetic basis of kidney cancer: a metabolic disease. Nat Rev Urol. 2010, 7 (5): 277-285. 10.1038/nrurol.2010.47.View ArticlePubMedPubMed CentralGoogle Scholar
- Baldewijns MM, van Vlodrop IJ, Vermeulen PB, Soetekouw PM, van Engeland M, de Bruine AP: VHL and HIF signalling in renal cell carcinogenesis. J Pathol. 2010, 221 (2): 125-138. 10.1002/path.2689.View ArticlePubMedGoogle Scholar
- Sudarshan S, Karam JA, Brugarolas J, Thompson RH, Uzzo R, Rini B, Margulis V, Patard JJ, Escudier B, Linehan WM: Metabolism of kidney cancer: from the lab to clinical practice. Eur Urol. 2013, 63 (2): 244-251. 10.1016/j.eururo.2012.09.054.View ArticlePubMedGoogle Scholar
- Selak MA, Armour SM, MacKenzie ED, Boulahbel H, Watson DG, Mansfield KD, Pan Y, Simon MC, Thompson CB, Gottlieb E: Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-alpha prolyl hydroxylase. Cancer Cell. 2005, 7 (1): 77-85. 10.1016/j.ccr.2004.11.022.View ArticlePubMedGoogle Scholar
- Linehan WM, Rouault TA: Molecular pathways: Fumarate hydratase-deficient kidney cancer–targeting the Warburg effect in cancer. Clin Cancer Res. 2013, 19 (13): 3345-3352. 10.1158/1078-0432.CCR-13-0304.View ArticlePubMedPubMed CentralGoogle Scholar
- Linehan WM, Ricketts CJ: The metabolic basis of kidney cancer. Semin Cancer Biol. 2013, 23 (1): 46-55. 10.1016/j.semcancer.2012.06.002.View ArticlePubMedGoogle Scholar
- Locasale JW: Serine, glycine and one-carbon units: cancer metabolism in full circle. Nat Rev Cancer. 2013, 13 (8): 572-583. 10.1038/nrc3557.View ArticlePubMedPubMed CentralGoogle Scholar
- Moore LE, Hung R, Karami S, Boffetta P, Berndt S, Hsu CC, Zaridze D, Janout V, Kollarova H, Bencko V, Navratilova M, Szeszenia-Dabrowska N, Mates D, Mukeria A, Holcatova I, Yeager M, Chanock S, Garcia-Closas M, Rothman N, Chow WH, Brennan P: Folate metabolism genes, vegetable intake and renal cancer risk in central Europe. Int J Cancer. 2008, 122 (8): 1710-1715.View ArticlePubMedGoogle Scholar
- Gibson TM, Brennan P, Han S, Karami S, Zaridze D, Janout V, Kollarova H, Bencko V, Navratilova M, Szeszenia-Dabrowska N, Mates D, Slamova A, Pfeiffer RM, Stolzenberg-Solomon RZ, Mayne ST, Yeager M, Chanock S, Rothman N, Chow WH, Rosenberg PS, Boffetta P, Moore LE: Comprehensive evaluation of one-carbon metabolism pathway gene variants and renal cell cancer risk. PLoS One. 2011, 6 (10): e26165-10.1371/journal.pone.0026165.View ArticlePubMedPubMed CentralGoogle Scholar
- Greenberg AK, Rimal B, Felner K, Zafar S, Hung J, Eylers E, Phalan B, Zhang M, Goldberg JD, Crawford B, Rom WN, Naidich D, Merali S: S-adenosylmethionine as a biomarker for the early detection of lung cancer. Chest. 2007, 132 (4): 1247-1252. 10.1378/chest.07-0622.View ArticlePubMedPubMed CentralGoogle Scholar
- de Vogel S, Schneede J, Ueland PM, Vollset SE, Meyer K, Fredriksen A, Midttun O, Bjorge T, Kampman E, Bretthauer M, Hoff G: Biomarkers related to one-carbon metabolism as potential risk factors for distal colorectal adenomas. Cancer Epidemiol Biomarkers Prev. 2011, 20 (8): 1726-1735. 10.1158/1055-9965.EPI-11-0359.View ArticlePubMedGoogle Scholar
- Lu SC, Mato JM: Role of methionine adenosyltransferase and S-adenosylmethionine in alcohol-associated liver cancer. Alcohol. 2005, 35 (3): 227-234. 10.1016/j.alcohol.2005.03.011.View ArticlePubMedGoogle Scholar
- Garcia-Trevijano ER, Latasa MU, Carretero MV, Berasain C, Mato JM, Avila MA: S-adenosylmethionine regulates MAT1A and MAT2A gene expression in cultured rat hepatocytes: a new role for S-adenosylmethionine in the maintenance of the differentiated status of the liver. FASEB J. 2000, 14 (15): 2511-2518. 10.1096/fj.00-0121com.View ArticlePubMedGoogle Scholar
- Hashimoto H, Vertino PM, Cheng X: Molecular coupling of DNA methylation and histone methylation. Epigenomics. 2010, 2 (5): 657-669. 10.2217/epi.10.44.View ArticlePubMedPubMed CentralGoogle Scholar
- Cedar H, Bergman Y: Linking DNA methylation and histone modification: patterns and paradigms. Nat Rev Genet. 2009, 10 (5): 295-304. 10.1038/nrg2540.View ArticlePubMedGoogle Scholar
- Ara AI, Xia M, Ramani K, Mato JM, Lu SC: S-adenosylmethionine inhibits lipopolysaccharide-induced gene expression via modulation of histone methylation. Hepatology. 2008, 47 (5): 1655-1666. 10.1002/hep.22231.View ArticlePubMedPubMed CentralGoogle Scholar
- Halim AB, LeGros L, Chamberlin ME, Geller A, Kotb M: Regulation of the human MAT2A gene encoding the catalytic alpha 2 subunit of methionine adenosyltransferase, MAT II: gene organization, promoter characterization, and identification of a site in the proximal promoter that is essential for its activity. J Biol Chem. 2001, 276 (13): 9784-9791. 10.1074/jbc.M002347200.View ArticlePubMedGoogle Scholar
- Liu Q, Liu L, Zhao Y, Zhang J, Wang D, Chen J, He Y, Wu J, Zhang Z, Liu Z: Hypoxia induces genomic DNA demethylation through the activation of HIF-1alpha and transcriptional upregulation of MAT2A in hepatoma cells. Mol Cancer Ther. 2011, 10 (6): 1113-1123. 10.1158/1535-7163.MCT-10-1010.View ArticlePubMedGoogle Scholar
- Zhang T, Zheng Z, Liu Y, Zhang J, Zhao Y, Liu Y, Zhu H, Zhao G, Liang J, Li Q, Xu H: Overexpression of methionine adenosyltransferase II alpha (MAT2A) in gastric cancer and induction of cell cycle arrest and apoptosis in SGC-7901 cells by shRNA-mediated silencing of MAT2A gene. Acta Histochem. 2013, 115 (1): 48-55. 10.1016/j.acthis.2012.03.006.View ArticlePubMedGoogle Scholar
- Chen H, Xia M, Lin M, Yang H, Kuhlenkamp J, Li T, Sodir NM, Chen YH, Josef-Lenz H, Laird PW, Clarke S, Mato JM, Lu SC: Role of methionine adenosyltransferase 2A and S-adenosylmethionine in mitogen-induced growth of human colon cancer cells. Gastroenterology. 2007, 133 (1): 207-218. 10.1053/j.gastro.2007.03.114.View ArticlePubMedGoogle Scholar
- Wang Q, Liu QY, Liu ZS, Qian Q, Sun Q, Pan DY: Inhibition of hepatocelluar carcinoma MAT2A and MAT2beta gene expressions by single and dual small interfering RNA. J Exp Clin Cancer Res. 2008, 27: 72-10.1186/1756-9966-27-72.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang W, Sviripa V, Chen X, Shi J, Yu T, Hamza A, Ward ND, Kril LM, Vander Kooi CW, Zhan CG, Evers BM, Watt DS, Liu C: Fluorinated N, N-dialkylaminostilbenes repress colon cancer by targeting methionine S-adenosyltransferase 2A. ACS Chem Biol. 2013, 8 (4): 796-803. 10.1021/cb3005353.View ArticlePubMedPubMed CentralGoogle Scholar
- Katoh Y, Ikura T, Hoshikawa Y, Tashiro S, Ito T, Ohta M, Kera Y, Noda T, Igarashi K: Methionine adenosyltransferase II serves as a transcriptional corepressor of Maf oncoprotein. Mol Cell. 2011, 41 (5): 554-566. 10.1016/j.molcel.2011.02.018.View ArticlePubMedGoogle Scholar
- Frezza C, Zheng L, Folger O, Rajagopalan KN, MacKenzie ED, Jerby L, Micaroni M, Chaneton B, Adam J, Hedley A, Kalna G, Tomlinson IP, Pollard PJ, Watson DG, Deberardinis RJ, Shlomi T, Ruppin E, Gottlieb E: Haem oxygenase is synthetically lethal with the tumour suppressor fumarate hydratase. Nature. 2011, 477 (7363): 225-228. 10.1038/nature10363.View ArticlePubMedGoogle Scholar
- Banerjee P, Basu A, Datta D, Gasser M, Waaga-Gasser AM, Pal S: The heme oxygenase-1 protein is overexpressed in human renal cancer cells following activation of the Ras-Raf-ERK pathway and mediates anti-apoptotic signal. J Biol Chem. 2011, 286 (38): 33580-33590. 10.1074/jbc.M111.248401.View ArticlePubMedPubMed CentralGoogle Scholar
- Banerjee P, Basu A, Wegiel B, Otterbein LE, Mizumura K, Gasser M, Waaga-Gasser AM, Choi AM, Pal S: Heme oxygenase-1 promotes survival of renal cancer cells through modulation of apoptosis- and autophagy-regulating molecules. J Biol Chem. 2012, 287 (38): 32113-32123. 10.1074/jbc.M112.393140.View ArticlePubMedPubMed CentralGoogle Scholar
- Miyata Y, Koga S, Kanda S, Nishikido M, Hayashi T, Kanetake H: Expression of cyclooxygenase-2 in renal cell carcinoma: correlation with tumor cell proliferation, apoptosis, angiogenesis, expression of matrix metalloproteinase-2, and survival. Clin Cancer Res. 2003, 9 (5): 1741-1749.PubMedGoogle Scholar
- Tuna B, Yorukoglu K, Gurel D, Mungan U, Kirkali Z: Significance of COX-2 expression in human renal cell carcinoma. Urology. 2004, 64 (6): 1116-1120. 10.1016/j.urology.2004.07.023.View ArticlePubMedGoogle Scholar
- Igarashi K, Katoh Y: Metabolic aspects of epigenome: coupling of S-Adenosylmethionine synthesis and gene regulation on chromatin by SAMIT module. Subcell Biochem. 2012, 61: 105-118.View ArticleGoogle Scholar
- Kurdistani SK: Histone modifications in cancer biology and prognosis. Prog Drug Res. 2011, 67: 91-106.PubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/14/196/prepub
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