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Ratio of n-3/n-6 PUFAs and risk of breast cancer: a meta-analysis of 274135 adult females from 11 independent prospective studies

BMC Cancer201414:105

DOI: 10.1186/1471-2407-14-105

Received: 16 December 2013

Accepted: 18 December 2013

Published: 18 February 2014

Abstract

Background

Increased ratio of n-3/n-6 polyunsaturated fatty acids (PUFAs) in diet or serum may have a protective effect on the risk of breast cancer (BC); however, the conclusions from prospective studies are still controversial. The purpose of this study is to ascertain the relationship between intake ratio of n-3/n-6 PUFAs and the risk of BC, and estimate the potential summarized dose–response trend.

Methods

Relevant English-language studies were identified through Cochrane Library, PubMed and EMBASE database till April 2013. Eligible prospective studies reporting the multivariate adjusted risk ratios (RRs) for association of n-3/n-6 PUFAs ratio in diet or serum with BC risk. Data extraction was conducted independently by 2 investigators; disagreements were reconciled by consensus. Study quality was assessed using the Newcastle-Ottawa scale. Study-specific RRs were combined via a random-effects model.

Results

Six prospective nested case–control and 5 cohort studies, involving 8,331 BC events from 274,135 adult females across different countries, were included in present study. Subjects with higher dietary intake ratio of n-3/n-6 PUFAs have a significantly lower risk of BC among study populations (pooled RR = 0.90; 95% CI: 0.82, 0.99), and per 1/10 increment of ratio in diet was associated with a 6% reduction of BC risk (pooled RR = 0.94; 95% CI: 0.90, 0.99; P for linear trend = 0.012). USA subjects with higher ratio of n-3/n-6 in serum phospholipids (PL) have a significantly lower risk of BC (pooled RR = 0.62; 95% CI: 0.39, 0.97; I 2 = 0.00%; P for metaregression = 0.103; P for a permutation test = 0.100), and per 1/10 increment of ratio in serum PL was associated with 27% reduction of BC risk (pooled RR = 0.73; 95% CI: 0.59, 0.91; P for linear trend = 0.004; P for metaregression = 0.082; P for a permutation test = 0.116).

Conclusions

Higher intake ratio of n-3/n-6 PUFAs is associated with lower risk of BC among females, which implies an important evidence for BC prevention and treatment is by increasing dietary intake ratio of n-3/n-6 PUFA. No firm conclusions from USA populations could be obtained, due to the limited numbers of USA studies.

Background

Although breast cancer (BC) is the most common cancer occurring among women worldwide, international variation of BC incidence show there is a higher incidence in North America and Western Europe, but lower incidence in Asia [1, 2]. The large geographic heterogeneity of incidence among women globally could be explained by variation of dietary patterns, especially with relation to dietary fat as a potential dietary factor that is closely correlated with increased incidence of BC [36]. Polyunsaturated fatty acids (PUFAs) as dietary fat subtypes consist of two families: n-3 PUFAs and n-6 PUFAs. Serum phospholipids (PL) ratio of n-3/n-6 PUFA can directly reflect dietary intake ratio of n-3/n-6 PUFA, due to the lack of interconversion between n-3 and n-6 PUFAs in humans. N-3 and n-6 PUFAs in vivo can influence breast tumor cell growth by simultaneously competing for the same metabolic pathway (COX and LOX pathway) to change the balance of tissue eicosanoids, the transcription mediated by nuclear factor κB (NF-κB), and signal transduction mediated by the mammalian target of rapamycin (mTOR) etc. [79]. Therefore, ratio of n-3/n-6 PUFAs in diet and serum PL probably plays an important role in the risk of BC.

The studies from cell lines and animals have shown promising results of down regulating BC tumor growth by n-3 PUFAs as a nutrient to compete with n-6 PUFAs [10, 11]. Most of the case–control studies also support that dietary or serum PL n-3/n-6 ratio is inversely associated with risk of BC [4, 5, 1214]. However, there are some inconsistent conclusions in prospective studies [1520], and the optimal intake ratio of n-3/n-6 PUFAs has not yet been well defined. Therefore, it is necessary to quantitatively ascertain the association between intake ratio of n-3/n-6 PUFAs and the risk of BC by means of meta-analysis. Available data from prospective studies of adult females (premenopausal, postmenopausal, or combined) across different countries were pooled to summarize the relationship between intake ratio of n-3/n-6 PUFAs and the risk of BC for highest vs. lowest quantile, to estimate the potential dose–response trend and to conduct the stratified analysis for exploring the probable source of heterogeneity.

Methods

Literature search

We identified prospective studies which reported the association between intake ratio of n-3/n-6 (n-6/n-3) and BC risk up until April 2013 from PubMed, Embase, and Cochrane Library database using literature retrieval of subject headings. Search strategy was (“Fatty Acids, Omega-3″ OR “Fatty Acids, Omega-6″ ) AND “Breast Neoplasms” for PubMed, “Breast tumor” AND (“omega 3 fatty acid” OR “omega 6 fatty acid”) for EMBASE and “Fatty Acids” AND “Breast Neoplasms” for Cochrane Library databases. We also searched systematic reviews from the above-mentioned database, and checked reference lists to identify studies that might have been missed. The present meta-analysis was conducted using the standard methods from Cochrane Collaboration, and reporting items were mainly based on MOOSE guidelines for meta-analysis of observational studies [21] (Additional file 1). Ethical approval and informed consent were not required for this meta-analysis.

Eligibility criteria

1) Participants: study population included any adult women (premenopausal, postmenopausal, or combined), whose base conditions were regarded as stable; 2) Exposure: evaluating ratio of n-3/n-6 (n-6/n-3) PUFAs in diet or human serum (plasma) PL; 3) Outcomes: evaluating BC incidence as outcome variable and providing risk ratios (RRs) for all categories of dietary or serum (plasma) PL ratio of n-3/n-6 or n-6/n-3 PUFAs; 4) Study Design: prospective studies (cohort, nested case–control and case-cohort study) were included.

Study identification

Two trained investigators (YF and JG) identified articles eligible for further review by performing a stepwise screening of titles or abstracts, followed by a full-text review based on common inclusion criterion. Discrepancies were resolved through discussion with the third investigator (BY). Studies of cross-sectional, cross-over, randomized controlled trials (RCT), experimental designs (cell culture and animal test), non- original research (reviews, editorials, or commentaries), abstract, unpublished studies, or duplicated studies were excluded. Our search was restricted to human studies published in English. We did not contact authors for the detailed information of primary studies only reporting association of n-3 or n-6 PUFA with BC risk. We contacted the authors of the two studies reporting association of n-3 and n-6 PUFA with BC risk by email.

Data extraction and quality assessment

Data extraction was finished independently and performed twice by two reviewers (YF and JG), and disagreements were reconciled by consensus. Detailed data concerning participants, exposure, comparability and outcomes were extracted using a standard extraction form (Additional file 2). We mainly aimed to extract the characteristics of participants (e.g., nationality, age, menopausal status, follow-up duration and number of participants), intake n-3/n-6 (n-6/n-3) ratio exposure (e.g., measurement method, exposure source, and exposure range), covariates adjusted in multivariable analysis and RRs including corresponding confidence intervals (CIs) for all categories of dietary or serum (plasma) PL ratio of n-3/n-6 (n-6/n-3) PUFAs. Quality assessment was performed by using the Newcastle-Ottawa scale (NOS) [22], which mainly contains selection domain (0–4 stars), comparability domain (0–2 stars) and exposure or outcomes domain (0–3 stars).

Data synthesis and statistic analysis

In this meta-analysis, intake ratio of n-3/n-6 PUFAs was defined as the proportion of total n-3 PUFAs (the sum of ALA, EPA and DHA) to total n-6 PUFAs in diet or serum (plasma) PL, and pooled RR including corresponding 95% CI was taken as the summary risk estimate for all studies. RRs from each study were firstly transformed to their logarithm (logRR), and corresponding 95% CIs were used to calculate corresponding standard errors (selogRR). We conducted two types of meta-analysis. Firstly, we conducted meta-analysis for the highest quantile (tertile, quartile and quintile) compared with lowest or reference, and study-specific RRs were combined using a random-effects model described by DerSimonian and Laird [23], which considers both within-study and between-study variability. Subsequently, summary dose–response meta-analysis was performed using the method described by Greenland and Orsini, et al. [24, 25] to estimate the potential linear trend and achieve association between per 1/10 increment of intake ratio of n-3/n-6 PUFAs and BC risk (Additional file 3). To examine a potential nonlinear (curvilinear) trend, we used the restricted cubic splines functional model with three knots at percentiles 25%, 50%, and 75% of the distribution. A p-value for curvilinear trend was calculated by testing the null hypothesis that the coefficient of the second spline is equal to zero [26, 27].

Heterogeneity was assessed with the Q test and I 2 statistic. We considered an I 2 value greater than 50% and 2-tailed P < 0.10 as indicative of heterogeneity according to Cochrane Handbook, and defined the low, moderate and high degrees of heterogeneity by I 2 values of 25%, 50% and 75% as cut-off points [28] respectively. If heterogeneity was presented in this meta-analysis, metaregression and subgroup analyses were conducted to identify the potential sources of heterogeneity by study design (cohort study and nested case–control study), different regions (Europe, USA and Asia), menopausal status (pre-, post- and combined), and follow-up duration (more than and less than average value) and known covariates adjusted (e.g., BMI, age, and family history of BC) in multivariate analysis.

Sensitivity analysis was performed to evaluate potential influence of individual study on overall risk estimation, and compare the pooled RR from random effect model with that from fixed effect model. Potential publication bias was qualitatively delineated by the asymmetry of funnel plot, and it was also quantitatively examined by Begg’s test and Egger’s regression test (P < 0.05 was considered representative of statistical significance) [29]. If potential publication bias was found in the meta-analyses, contour-enhanced funnel plot was performed to explore the probable source of publication bias [30]. This method examined the visual asymmetry of funnel plot, and differentiated asymmetry due to publication bias from other factors [31]. Statistical analysis of the combined data was performed by STATA version 11.0 (Stata CORP, College Station, TX).

Results

We identified 1,112 potential studies from electronic search, and 772 studies were left after removing duplicates. Thirty one prospective studies were obtained after title and abstract review. Eleven studies were eligible for inclusion in the present study after full text review (Figure 1), and 20 studies were excluded for other reasons (Additional file 3).
Figure 1

PRISMA flow diagram for included prospective studies.

Characteristics of the included studies

Included studies consist of 5 prospective cohort studies [1517, 32, 33] and 6 prospective nested case–control studies [1820, 3436] (Table 1; Additional file 1). The 6 studies looked at intake of dietary fatty acid [1517, 32, 33, 35], which was quantified by food frequency questionnaires using the measurement grams per day (g/d) [16, 33, 35], percentage of energy (% energy) [17, 32], and grams per 1,000 kilocalorie (g/1000 kcal) [15]. There are 5 studies concerning serum PL biomarker, where fatty acid compositions in serum PL was quantified by gas chromatography, and measurement unit was percentage of total fatty acids, except for 1 study (mg/L) [18]. One study provided data of pre- and post-menopausal women separately [19], 1 study of pre-menopausal [18], 4 studies of post-menopausal [15, 33, 35, 36], and 5 studies of combined women [16, 17, 30, 32, 34]. Five studies were reported from Europe [18, 20, 32, 34, 35], 4 studies from USA [15, 19, 33, 36], and 2 studies from Asia [16, 17]. NOS stars of all included studies ranged from 4 to 10, with an average of 7.55. High quality studies (NOS stars ≥ 8) accounted for 55% of all studies [1517, 19, 33, 36], and moderate quality studies (6 ≤ NOS stars ≤ 7) accounted for 45% of all studies [18, 20, 32, 34, 35].
Table 1

Characteristics of included prospective studies

Study (nation)

Design

Population (case/participants)

Menopausal status

Follow-up duration (years)

Exposure

Outcomes (RRs, 95% CI)

Study quality

Measurement

Range (H vs. L)a

Vatten 1993 [18] (Norway)

NCC

Subjects from serum bank; 87/235;

Pre-

5

n-3/n-6: Serum PL, GC (mg/L)

0.36 vs. 0.14

1.0 (0.4, 2.1)

Moderate

Chajes 1999 [35] (Sweden)

NCC

Cardiovascular disease Cohort; 196/388;

Combined

2 ~ 11

LC n-3/n-6: Serum PL, GC (%tFC)

> 0.68 vs. <0.08

0.88 (0.42,1.86)

Moderate

Saadatian-Elahi 2002 [19] (USA)

NCC

Health university Women cohort; 197/197;

Pre- and Post-

4.3

n-3/n-6: Serum PL, GC (%tFC)

4th quantile vs. reference

Pre-: 0.60 (0.24, 1.54);

High

Post-: 0.42 (0.17, 1.08)

Wirfalt 2002 [36] (Sweden)

NCC

Malmo Diet and Cancer (MDC) Cohort; 237/673;

Post-

3 ~ 8

n-3/n-6: Diet, FFQ (g/day)

0.33 vs. 0.15

0.66 (0.41, 1.08)

Moderate

Wakai 2005 [17] (Japan)

PC

Japan Collaborative Cohort Study (JACC);129/26291

Post- and Combined

7.6

n-6/n-3: Diet, FFQ (% energy)

Combined: > 4.61vs. < 3.25

Combined: 1.31 (0.78, 2.19)

High

Post-: > 4.59 vs. < 3.21

Post-: 1.30 (0.66, 2.58)

Chajes 2008 [20] (Sweden)

NCC

Europe Prospective Investigation into Cancer and Nutrition (EPIC); 363/702;

Combined.

7.0

n-6/n-3: Serum PL, GC (%tFC)

5th quantile vs. reference

0.76 (0.48, 1.20)

Moderate

Takata 2009 [37] (USA)

NCC

Beta Carotene and Retinol Efficacy Trial chort study (CARET); 103/309;

Post-.

4.4

n-3/n-6: Serum PL, GC (%tFC)

> 0.15 vs. < 0.11

0.74 (0.40, 1.36)

High

Thiebaut 2009 [33] (France)

PC

EPIC Cohort; 1650/56007

Combined

8.0

n-6/n-3:Diet, FFQ, (% energy)

14.76 vs. 5.48

0.97 (0.83, 1.14)

Moderate

Murff 2011 [16] (China)

PC

Shanghai Women Health Study cohort (SWHS); 712/72571;

Combined

8.0

n-6/n-3: Diet, FFQ, (g/day)

7.64 vs.5.18

1.02 (0.77, 1.34)

High

Park 2012 [15] (USA)

PC

Multiethnic Cohort; 3885/85089;

Post-

12

n-6/n-3: Diet, FFQ, (g/1000 kcal)

> 9.60 vs. < 7.60

1.10 (0.99, 1.22)

High

Sczaniecka 2012 [34] (USA)

PC

Vitamins and Lifestyle (VITAL) cohort study; 772/30252;

Post-.

6.0

n-3/n-6: Diet, FFQ, (g/day)

> 0.03 vs. < 0.005

0.84 (0.65, 1.09)

High

        

aH vs. L: the highest exposure quantile vs. lowest or reference.

%tFC = percentage of total Fatty Acid; GC = Gas Chromatography; LC n-3 = long chain n-3 PUFAs including EPA, DPA and DHA; NCC = prospective nested case–control study; PC = prospective cohort study; n-3/n-6 = ratio of n-3/n-6 polyunsaturated fatty acids; n-6/n-3 = ratio of n-6/n-3 polyunsaturated fatty acids; FFQ = food frequency questionnaire; Serum PL = serum phospholipids.

Highest vs. lowest quantile of ratio of n-3/n-6 PUFAs

We performed a random-effects model meta-analysis for highest quantile compared with lowest (Figure 2). Eleven independent prospective studies reported the association between ratio of n-3/n-6 and risk of breast cancer, involving 8,331 BC events and 274,135 adult females (premenopausal, postmenopausal, or combined) across different countries. Intake ratio of n-3/n-6 PUFA was inversely associated with BC risk for the highest vs. lowest quantile among study populations (pooled RR = 0.90; 95% CI: 0.82, 0.99; I 2 = 11.40%; P heterogeneity = 0.33).
Figure 2

Forest plot of ratio of n-3/n-6 PUFAs in diet or serum PL for the highest vs. lowest. Prospective studies concerning dietary and serum PL ratio of n-3/n-6 PUFAs separately are referred to by first author, year of publication and number of subjects, weighted and ranked according to the inverse of the variance of the logRR estimate. The relative risks (RRs) are represented by the squares (the size is proportional to the weights used in the meta-analysis), and CIs are represented by the error bars. P values for heterogeneity test (I square and Q test) and RR for the highest exposure quantile vs. lowest from individual study were pooled by using random effect model. The diamonds can represent the pooled RR from subtotal risk estimate of dietary or serum PL ratio, according to their corresponding position in the figure.

Summarized dose–response meta-analysis

We performed summarized dose–response meta-analysis to determine the potential linear and curvilinear trend. Four articles reporting PUFA as g/day were eligible for the dose–response association between dietary ratio and BC risk [15, 16, 33, 35]. There was statistical significance of dose–response trend (P linear = 0.012; P curvilinear = 0.018) among study populations. Per 1/10 increment of dietary n-3/n-6 ratio was associated with a 6% reduction of BC risk (pooled RR = 0.94; 95% CI: 0.90, 0.99; I 2 = 3.20%, P heterogeneity = 0.38) (Figures 3 & 4).
Figure 3

Forest plot of per 1/10 increment of n-3/n-6 PUFAs ratio in diet and serum PL. Prospective studies eligible for dose–response analysis are referred to by first author and year of publication. The relative risks (RRs) are represented by the squares (the size is proportional to the weights used in the meta-analysis), and confidence intervals (CIs) are represented by the error bars. P values and I square for heterogeneity test were shown by using random effect model. The diamonds can separately represent the pooled RR for association between per 1/10 increment of dietary or serum PL ratio of n-3/n-6 PUFA and BC risk, which was combined by a two-stage random-effect model. Figure A indicated association of BC risk with per 1/10 dietary ratio of n-3/n-6 PUFA, whereas Figure B indicated association of BC risk with pre 1/10 serum PL ratio of n-3/n-6 PUFA.

Figure 4

Summarized dose–response association between dietary ratio of n-3/n-6 PUFAs and risk of breast cancer. Adjusted RRs from each exposure quantile of dietary ratio of n-3/n-6 PUFAs in included individual studies were represented by the gray diamonds, and corresponding intervals (CIs) were represented by the lightgray trendline. The dash line indicated that dose–response linear trend (P trend = 0.012) between dietary ratio of n-3/n-6 PUFAs and risk of breast cancer by use of variance-weighted least squares regression of fixed effect model; the black curve indicated nonlinear (curvilinear) trend (P trend = 0.018) by use of restricted cubic splines functional model with three knots at percentiles 25%, 50%, and 75% of the distribution.

Four articles reporting PUFAs as compositions were eligible for the dose–response association of serum PL biomarker with BC risk [15, 16, 18, 19, 3336]. There was no statistical significance of dose–response trend (P linear = 0.178; P curvilinear = 0.832) among study populations, and the pooled RR for per 1/10 increment of n-3/n-6 ratio in serum PL was 0.89 (95% CI: 0.74, 1.06; I 2 = 42.00%, P heterogeneity = 0.14) (Figure 3). Further stratifying by geographical regions, there was statistical significance of dose–response trend among USA populations from 2 studies (P linear = 0.004; P curvilinear = 0.09), and per 1/10 increment of n-3/n-6 ratio in serum PL was associated with a 27% reduction of BC risk (pooled RR = 0.73; 95% CI: 0.59, 0.91; I 2 = 0.00%). There was no statistical significance of dose–response trend among Europe populations from 2 studies (P linear = 0.70; P curvilinear = 0.95), and the pooled RR of per 1/10 increment of ratio in serum PL was 1.03 (95% CI: 0.88, 1.21; I 2 = 0.00%) (Figure 3; Additional file 3). However, a permutation test from metaregression did not show significant difference between the two populations (P for metaregression = 0.086; P for a permutation test = 0.116).

Subgroup analysis of ratio of n-3/n-6 PUFA for highest quantile vs. lowest

Metaregression and subgroup analysis was performed to explore the probable source of heterogeneity (Table 2). Although the association of increased ratio of n-3/n-6 with decreased risk of BC among 98862 participants from 2 Asian studies was more evident than that among 116,147 participants from 4 USA studies (pooled RR = 0.88; 95% CI: 0.80, 0.97; I 2 = 0.00%) and 59,125 participants form 5 European studies (pooled RR = 1.00; 95% CI: 0.84, 1.18; I 2 = 8.90%), results of metaregression did not show a significant difference between the three populations. When stratified by the adjustment for potential confounders, metaregression did not show a significant difference between estimates adjusted and those not adjusted for specific covariates.
Table 2

Subgroup analysis of intake n-3/n-6 PUFAs ratio for highest quantile vs. lowest

Subgroup factors

N

Pooled RR (95% CI)

Heterogeneity

P b

P c

I 2

Degree

P a

Overall analysis

11

0.90 (0.82, 0.99)

11.40%

Low

0.33

  

Study design

     

0.53

0.60

PC

5

0.92 (0.84, 1.00)

7.70%

Low

0.37

  

NCC

6

0.82 (0.62, 1.08)

20.60%

Low

0.27

  

Nations

     

0.07

0.12

Europe

5

1.00 (0.84, 1.18)

8.90%

Low

0.36

  

USA

4

0.88 (0.80, 0.97)

0.00%

Low

0.95

  

Asia

2

0.77 (0.59, 1.00)

0.00%

Low

0.42

  

Menopausal status

     

0.18

0.26

Pre-

2

0.80 (0.43,1.48)

0.00%

Low

0.42

  

Post-

5

0.85 (0.75.0.97)

0.00%

Low

0.33

  

Combined

5

0.96 (0.80, 1.14)

23.30%

Low

0.27

  

Exposure assessment

     

0.87

0.99

Serum PL biomarker

5

0.86 (0.63, 1.20)

21.50%

Low

0.27

  

Diet

6

0.90 (0.82, 0.99)

17.30%

Low

0.30

  

Follow-up duration

     

0.11

0.08

≤ Average value

6

0.77 (0.46, 0.94)

0.00%

Low

0.77

  

> Average value

5

0.94 (0.83, 1.06)

19.50%

Low

0.21

  

Covariates adjusted

       

BMI

     

0.24

0.36

Yes

8

0.91 (0.83, 1.00)

9.90%

Low

0.35

  

No

3

0.65 (0.39,1.09)

0.00%

Low

0.38

  

Age of fist childbirth

     

0.09

0.07

Yes

7

0.87 (0.79, 0.94)

0.00%

Low

0.56

  

No

4

1.01 (0.70, 1.12)

0.00%

Low

0.54

  

Age at menarche

     

0.51

0.54

Yes

6

0.92 (0.85, 0.99)

0.00%

Low

0.51

  

No

5

0.80 (0.58,1.11)

33.60%

Moderate

0.18

  

Parity

     

0.11

0.08

Yes

5

0.94 (0.85, 1.04)

15.90%

Low

0.31

  

No

6

0.77 (0.63, 0.94)

0.00%

Low

0.68

  

Reproductive variables

     

0.65

0.76

Yes

4

0.89 (0.81, 0.98)

0.00%

Low

0.73

  

No

7

0.89 (0.74, 1.07)

33.70%

Moderate

0.16

  

Family history of BC

     

0.30

0.30

Yes

8

0.91 (0.81, 1.02)

31.30%

Moderate

0.18

  

No

3

0.76 (0.56,1.04)

0.00%

Low

0.83

  

Hormone user

     

0.75

0.90

Yes

9

0.89 (0.80, 1.00)

24.50%

Low

0.22

  

No

2

0.82 (0.50, 1.35)

0.00%

Low

0.57

  

Alcohol intake

     

0.32

0.32

Yes

9

0.91 (0.82, 1.00)

0.00%

Low

0.26

  

No

2

0.72 (0.47, 1.10)

20.60%

Low

0.51

  

Smoking

     

0.23

0.28

Yes

4

0.94 (0.86, 1.02)

0.00%

Low

0.40

  

No

7

0.83 (0.70, 0.98)

8.20%

Low

0.37

  

Age

     

0.78

0.99

Yes

6

0.92 (0.82, 1.02)

0.00%

Low

0.45

  

No

5

0.86 (0.68, 1.10)

34.00%

Moderate

0.18

  

Educational status

     

0.16

0.14

Yes

5

0.87 (0.80, 0.96)

0.00%

Low

0.59

  

No

6

0.96 (0.79, 1.17)

14.70%

Low

0.32

  

Total energy intake

     

0.16

0.16

Yes

5

0.87 (0.80, 0.96)

0.00%

Low

0.59

  

No

6

0.96 (0.79, 1.17)

14.70%

Low

0.32

  

Physical activity

     

0.20

0.20

Yes

3

0.81 (0.67, 0.97)

0.00%

Low

0.90

  

No

8

0.92 (0.81, 1.05)

21.50%

Low

0.25

  

Other drugs or nutrients intake

     

0.20

0.24

Yes

3

0.81 (0.67, 0.97)

0.00%

Low

0.90

  

No

8

0.92 (0.81, 1.05)

21.50%

Low

0.25

  

a P: P value for heterogeneity within subgroup.

b P: P value for heterogeneity between subgroups with a meta-regression analysis.

c P: adjusted P value for heterogeneity between subgroups with a permutation test.

N = number of studies.

When separately analyzed by exposure assessment, we found a significantly negative association between BC risk and n-3/n-6 ratio in diet from 5 prospective cohort and 1 nested case–control studies, involving 7,385 BC invents and 271,357 participants (pooled RR = 0.92; 95% CI: 0.84, 1.00), whereas no significant association was found between BC risk and serum PL biomarker from 5 prospective nested case–control studies, involving 946 BC cases and 1832 controls (pooled RR = 0.86; 95% CI: 0.63, 1.20). However, there was no apparent difference between studies using diet ratio of n-3/n-6 as exposure and those using serum biomarker of n-3/n-6 as exposure with metaregression (P for metaregression = 0.870; P for a permutation test = 0.990).

Sensitivity analysis and publication bias

Finally, we conducted sensitivity analysis and publication bias analysis (Table 3). For dietary n-3/n-6 PUFAs ratio as exposure, sensitivity analysis indicated that exclusion of any individual study did not substantially change the end results. However, for serum PL biomarker, the sensitivity analysis after sequentially omitting 1 study at a time and reanalyzing the remaining data showed that there was significantly negative association between serum PL n-3/n-6 ratio and BC risk (pooled RR = 0.73; 95% CI: 0.51, 1.01; I 2 = 0.00%) after excluding the study by Chajes [20], indicating the overall risk estimation was substantially influenced by the single study [20].
Table 3

Sensitivity analysis for the relationship between intake n-3/n-6 PUFAs ratio and breast cancer risk

Sensitivity analysis

Diet ratio of n-3/n-6

Serum PL ratio of n-3/n-6

Intake ratio of n-3/n-6

N

Pooled RR (95% CI)

Heterogeneity

N

Pooled RR(95% CI)

Heterogeneity

N

Pooled RR(95% CI)

Heterogeneity

 

I 2 Degree

P

I 2 Degree

P

I 2 Degree

P

Exclusion of studies with potential selection bias [20, 32]

5

0.87 (0.80, 0.96)

0.00% (Low)

0.5s9

4

0.73 (0.51, 1.01)

0.00% (Low)

0.67

9

0.86 (0.79, 0.94)

0.00% (Low)

0.72

Exclusion of studies without covariates adjusted [18]

6

0.90 (0.82, 0.99)

17.30% (Low)

0.30

4

0.82 (0.56, 1.22)

36.50% (Low)

0.17

10

0.89 (0.81, 0.99)

19.10% (Low)

0.26

Contour-enhanced funnel plots of fixed effect model

6

0.91 (0.84, 0.98)

17.30% (Low)

0.30

5

0.90 (0.68, 1.19)

21.50% (Low)

0.27

11

0.91 (0.84, 0.98)

11.40% (Low)

0.33

Contour-enhanced funnel plots of random effect model

6

0.90 (0.82, 0.99)

17.30% (Low)

0.30

5

0.87 (0.63, 1.20)

21.50% (Low)

0.27

11

0.90 (0.82, 0.98)

11.40% (Low)

0.33

N = number of studies; P = P value for heterogeneity within subgroup.

In publication bias analysis, visual inspection of Begg’s funnel plot (P = 0.244) and Egger’s regression test (P = 0.138) showed no evidence of possible publication bias. Contour-enhanced funnel plots of random effect model showed studies appear to be missing in areas of high statistical significance (p < 0.05), indicating that publication bias is a less likely cause of the funnel asymmetry (Additional file 3).

Discussion

The present study, involving 8,331 BC events from 274,135 participants, indicated that higher ratio of n-3/n-6 PUFAs is associated with lower risk of BC, and per 1/10 increment of n-3/n-6 ratio in diet is associated with a 6% reduction of BC risk. However, subgroup analysis showed there was no significant relationship between serum PL biomarker and BC risk, but the significant difference between association of dietary n-3/n-6 ratio and serum PL with BC risk was not observed with metaregression (P for metaregression = 0.87; P for a permutation test = 0.99). Fatty acid profile in serum phospholipids may be an untypical representative of sensitive biomarkers indicating post-absorptive amounts and change at the target tissue, which did not provide the same information as the dietary questionnaire tools. Therefore, the strength of the relationship between the ratio in serum PL and BC risk might be lower compared with the relationship between the ratio in diet and the risk of BC.

Although the was no significant difference between most subgroups with metaregression, study heterogeneity (I 2 = 21.00%) in this meta-analysis indicated the subtotal variation was attributable to between individual studies. There are some possible reasons for explaining the potential heterogeneity in present study. Firstly, the heterogeneity from geographic regions could be partially explained by different dietary patterns. The n-3/n-6 ratio in Japanese diet is about 1 to 4, whereas that in western diet (USA and Europe) is about 1 to 15–20 [37]. Imbalance of n-3/n-6 PUFAs ratio in diet across different countries is the consequence of excessive n-6 PUFAs consumption largely from corn, sunflower or safflower oils in the Western diet, but higher n-3 PUFAs intake largely from marine foods in the Asian diet, particularly in the Japanese population, which partially explains the inconsistency between Asia and Western studies in this meta-analysis. Subsequently, lower n-3/n-6 ratio in the diet will lead to lower ratio of n-3/n-6 PUFAs in serum PL. One randomized clinical trial showed that plasma n-3/n-6 ratio significantly increased among subjects after lovaza intervention (a prescription-strength pill, 4 g/day, EPA + DHA = 3.36 g, duration for 2 years) compared with subjects without intervention [38]. In vivo, AA (20:4n-6, arachidonic acid) and EPA (20:5n-3, eicosapentaenoic acid) can simultaneously compete for the same cyclooxygenases (COX) and lipoxygenases (LOX) metabolism pathways, leading to the production of n-6 family derived 2-series PG and 4-series LT with promoting tumor growth effects [39, 40], and n-3 family derived 3-series PG and 5-series LT with suppressive effects [41, 42]. Thus, the higher ratio of n-3/n-6 PUFA entering the cellular pool from dietary sources could be involved in BC carcinogenesis by changing the balance of tissue eicosanoids. Finally, there is evidence that change of estrogen metabolism is probably involved in mammary carcinogenesis among post-menopausal females[43]. EPA/AA ratio present in cell membrane lipids could influence the balance of prostaglandin E 3 (PGE3)/prostaglandin E2 (PGE2) to inactivate the activity of adipose aromatase P 450 which catalyzes the conversion of 19-carbon steroids to estrogens, and thus reduce estrogen-stimulated cell growth action [44, 45].

This meta-analysis had several strengths. Firstly, the quantitative assessment was based on data from prospective cohort studies. This minimizes the possibility that overall analysis will be influenced due to recall bias, which could be of more common concern in retrospective case–control studies. Also, we had higher statistical power to estimate the relationship between intake ratio of n-3/n-6 PUFA and risk of BC by analyzing summary data from 274,135 participants in 11 prospective studies. Subsequently, serum PL as a biomarker directly reflecting intake ratio of n-3/n-6 fatty acids has the advantage of providing more objective ratio of n-3/n-6 PUFA in vivo, independent of subjective recall bias and probable measurable errors owing to dietary questionnaire tools used. Finally, more than 50% of the studies were high quality studies (NOS stars ≥ 8), and exclusion of any individual study did not significantly change the negative relationship between intake ratio of n-3/n-6 PUFAs and BC risk. Although publication bias could be of concern because small studies with null results tend not to be published, there was no evidence of potential publication bias in the present meta-analysis.

Meta-analyses of observational studies are susceptible to methodological and confounding biases inherent in the original studies. Therefore, there are also several limitations considered in our study. Firstly, although prospective studies are more superior to retrospective case–control studies with regards to elucidating causal relationships, selection bias from study populations might be still unavoidable. Volunteers recruited from occupational exposure populations (e.g., teacher, workers and nurse) might not be an unbiased representative of study population. Subsequently, although the fatty acid profile in serum PL, implying absorption and circulation transportation mechanisms, reflects acute dietary intakes over the past few days, it may be a untypical representation of sensitive biomarkers indicating post-absorptive amounts and change at the target tissue. Moreover, any measurement error and resulting misclassification would most likely lead to an attenuation of the true association. Intake of fatty acids was evaluated using dietary questionnaire tools, which could potentially have measurable errors, due to inaccurate fatty acids database and diet reporting bias. Similarly, fatty acids in serum PL,, especially long chain PUFAs, are probably undergoing change during the long follow-up time and storage time [46], due to double bonds being easily oxidized. Consequently, potential measurement bias might lead to underestimation or overestimation of risk of BC [47]. Although only original studies with adjusting for at least three covariates were included in the present study, the possibility of altering summary results from residual confounding factors still cannot be excluded. Data extraction and analyses were not blinded to the authors and publication agencies, and the references screening and data extraction were conducted independently and in duplicate by 2 investigators, therefore data selection bias was unlikely. Although reporting bias might be present in our study, due to English language bias from exclusion of non-English language articles, our eligible studies covered a wide range of non-English countries, such as countries across Europe and Asia, which partially eliminated the possible effects of report bias on overall risk estimates. Finally, the limited numbers of studies included in each subgroup might diminish the statistical power to detect the association between ratio of n-3/n-6 PUFA and BC risk. Although we separately made a stratified analysis for association of serum PL ratio with BC risk and dose–response trend, it is difficult to find firm evidences among study populations within subgroup analysis, particular among USA females (P for metaregression = 0.103; P for a permutation test = 0.100).

The present meta-analysis has also important medical implications of balanced n-3/n-6 fatty acid intake ratio for BC prevention, clinical diagnosis and treatment. Firstly, although many sensitive tumor biomarkers were used for the clinical diagnosis of BC, several potential limitations were still unavoidable. In addition, the BC inflammatory microenvironment plays a major role in growth, invasiveness and resistance to therapy. However, modulation ratio of n-3/n-6 fatty acids in food, as a potent inducer of metabolic responses, could provide an effective means to alter ratio of n-3/n-6 fatty acid in tumor tissue and thereby possibly affect tumor growth, by sensitizing cancer cells to chemotherapy and increasing resistance of normal cells to the toxic effects. Finally, diet intervention could represent a clinically relevant adjuvant therapy in patients with BC, based on the general tenet of clinical nutrition.

Conclusions

In summary, findings from this meta-analysis of prospective studies provide a conclusive evidence to support increase intake ratio of n-3/n-6 PUFAs for BC prevention among study females. Quantitative conclusions from the present study showed that per 1/10 increment of n-3/n-6 ratio in diet, there was a 6% reduction of BC risk among study populations (USA, Europe and Asia). This important evidence highlights the need to promote nutritional education programs, stressing the need to increase the consumption of food rich in n-3 PUFAs (marine foods), decrease the consumption of food rich in n-6 PUFAs (vegetable oils and processed foods), to ultimately improve intake ratio of n-3/n-6 PUFAs. However, no conclusive evidence from USA populations can be obtained and this needs to be,further confirmed by prospective population-based study on tissue biomarker of n-3/n-6 PUFAs ratio, and larger randomized controlled trials are required to determine whether higher intake ratio of n-3/n-6 PUFAs will have beneficial effects on BC risk, or improve the prognosis of patients with BC.

Declarations

Acknowledgements

The project was supported by the National Natural Science Foundation of China (No: 81273054), the National Basic Research Program of China (NO: 2011CB504002), and the PhD Programs Foundation of Ministry of Education of China (No: 20120101110107).

Authors’ Affiliations

(1)
Department of Food Science and Nutrition, Zhejiang University
(2)
Department of Preventive Medicine, Wenzhou Medical University
(3)
Medical Laboratory Animal Center, Wenzhou Medical University

References

  1. Parkin DM, Bray F, Ferlay J, Pisani P: Estimating the world cancer burden: Globocan 2000. Int J Cancer. 2001, 94 (2): 153-156. 10.1002/ijc.1440.View ArticlePubMedGoogle Scholar
  2. Bhoo-Pathy N, Yip CH, Hartman M, Uiterwaal CS, Devi BC, Peeters PH, Taib NA, van Gils CH, Verkooijen HM: Breast cancer research in Asia: adopt or adapt Western knowledge?. Eur J Cancer. 2013, 49 (3): 703-709. 10.1016/j.ejca.2012.09.014.View ArticlePubMedGoogle Scholar
  3. Shimizu H, Ross RK, Bernstein L, Yatani R, Henderson BE, Mack TM: Cancers of the prostate and breast among Japanese and white immigrants in Los Angeles County. Br J Cancer. 1991, 63 (6): 963-966. 10.1038/bjc.1991.210.View ArticlePubMedPubMed CentralGoogle Scholar
  4. Kim EH, Willett WC, Colditz GA, Hankinson SE, Stampfer MJ, Hunter DJ, Rosner B, Holmes MD: Dietary fat and risk of postmenopausal breast cancer in a 20-year follow-up. Am J Epidemiol. 2006, 164 (10): 990-997. 10.1093/aje/kwj309.View ArticlePubMedGoogle Scholar
  5. Zhang CX, Ho SC, Lin FY, Chen YM, Cheng SZ, Fu JH: Dietary fat intake and risk of breast cancer: A case–control study in China. Eur J Cancer Prev. 2011, 20 (3): 199-206. 10.1097/CEJ.0b013e32834572bb.View ArticlePubMedGoogle Scholar
  6. MacLennan M, Ma DWL: Role of dietary fatty acids in mammary gland development and breast cancer. Breast Cancer Res. 2010, 12 (5): 211-221. 10.1186/bcr2646.View ArticlePubMedPubMed CentralGoogle Scholar
  7. Karmali RA: Eicosanoids in neoplasia. Prev Med. 1987, 16 (4): 493-502. 10.1016/0091-7435(87)90063-6.View ArticlePubMedGoogle Scholar
  8. Wen ZH, Su YC, Lai PL, Zhang Y, Xu YF, Zhao A, Yao GY, Jia CH, Lin J, Xu S, Wang L, Wang XK, Liu AL, Jiang Y, Dai YF, Bai XC: Critical role of arachidonic acid-activated mTOR signaling in breast carcinogenesis and angiogenesis. Oncogene. 2013, 32 (2): 160-170. 10.1038/onc.2012.47.View ArticlePubMedGoogle Scholar
  9. Vendramini-Costa DB, Carvalho JE: Molecular link mechanisms between inflammation and cancer. Curr Pharm Des. 2012, 18 (26): 3831-3852. 10.2174/138161212802083707.View ArticlePubMedGoogle Scholar
  10. Chamras H, Ardashian A, Heber D, Glaspy JA: Fatty acid modulation of MCF-7 human breast cancer cell proliferation, apoptosis and differentiation. J Nutr Biochem. 2002, 13 (12): 711-716. 10.1016/S0955-2863(02)00230-9.View ArticlePubMedGoogle Scholar
  11. Stillwell W, Shaikh SR, Zerouga M, Siddiqui R, Wassall SR: Docosahexaenoic acid affects cell signaling by altering lipid rafts. Reprod Nutr Dev. 2005, 45 (5): 559-579. 10.1051/rnd:2005046.View ArticlePubMedGoogle Scholar
  12. Shannon J, King IB, Lampe JW, Gao DL, Ray RM, Lin MG, Stalsberg H, Thomas DB: Erythrocyte fatty acids and risk of proliferative and nonproliferative fibrocystic disease in women in Shanghai. China. Am J Clin Nutr. 2009, 89 (1): 265-276.View ArticlePubMedGoogle Scholar
  13. Kuriki K, Hirose K, Wakai K, Matsuo K, Ito H, Suzuki T, Hiraki A, Saito T, Iwata H, Tatematsu M, Tajima K: Breast cancer risk and erythrocyte compositions of n-3 highly unsaturated fatty acids in Japanese. Int J Cancer. 2007, 121 (2): 377-385. 10.1002/ijc.22682.View ArticlePubMedGoogle Scholar
  14. Maillard V, Bougnoux P, Ferrari P, Jourdan ML, Pinault M, Lavillonniere F, Body G, Le Floch O, Chajes V: N-3 and N-6 fatty acids in breast adipose tissue and relative risk of breast cancer in a case–control study in Tours. France. Int J Cancer. 2002, 98 (1): 78-83. 10.1002/ijc.10130.View ArticlePubMedGoogle Scholar
  15. Park SY, Kolonel LN, Henderson BE, Wilkens LR: Dietary fat and breast cancer in postmenopausal women according to ethnicity and hormone receptor status: the multiethnic cohort study. Cancer Prev Res (Phila). 2012, 5 (2): 216-228. 10.1158/1940-6207.CAPR-11-0260.View ArticleGoogle Scholar
  16. Murff HJ, Shu XO, Li HL, Yang G, Wu XY, Cai H, Wen WQ, Gao YT, Zheng W: Dietary polyunsaturated fatty acids and breast cancer risk in Chinese women: a prospective cohort study. Int J Cancer. 2011, 128 (6): 1434-1441. 10.1002/ijc.25703.View ArticlePubMedGoogle Scholar
  17. Wakai K, Tamakoshi K, Date C, Fukui M, Suzuki S, Lin Y, Niwa Y, Nishio K, Yatsuya H, Kondo T, Tokudome S, Yamamoto A, Toyoshima H, Tamakoshi A, JACC Study Group: Dietary intakes of fat and fatty acids and risk of breast cancer: A prospective study in Japan. Cancer Sci. 2005, 96 (9): 590-599. 10.1111/j.1349-7006.2005.00084.x.View ArticlePubMedGoogle Scholar
  18. Vatten LJ, Bjerve KS, Andersen A, Jellum E: Polyunsaturated fatty acids in serum phospholipids and risk of breast cancer: a case–control study from the Janus serum bank in Norway. Eur J Cancer. 1993, 29A (4): 532-538.View ArticlePubMedGoogle Scholar
  19. Saadatian-Elahi M, Toniolo P, Ferrari P, Goudable J, Akhmedkhanov A, Zeleniuch-Jacquotte A, Riboli E: Serum fatty acids and risk of breast cancer in a nested case–control study of the New York University Women’s Health Study. Cancer Epidem Biomar. 2002, 11 (11): 1353-1360.Google Scholar
  20. Chajes V, Thiebaut ACM, Rotival M, Gauthier E, Maillard V, Boutron-Ruault MC, Joulin V, Lenoir GM, Clavel-Chapelon F: Association between serum trans-monounsaturated fatty acids and breast cancer risk in the E3N-EPIC study. Am J Epidemiol. 2008, 167 (11): 1312-1320. 10.1093/aje/kwn069.View ArticlePubMedPubMed CentralGoogle Scholar
  21. Stroup DF, Berlin JA, Morton SC, Olkin I, Williamson GD, Rennie D, Moher D, Becker BJ, Sipe TA, Thacker SB: Meta-analysis of observational studies in epidemiology: a proposal for reporting. Meta-analysis Of Observational Studies in Epidemiology (MOOSE) group. JAMA. 2000, 283 (15): 2008-2012. 10.1001/jama.283.15.2008.View ArticlePubMedGoogle Scholar
  22. Wells GA SB, O'Connell D, Peterson J, Welch V, Losos M: The Newcastle-Ottawa Scale (NOS) for assessing the quality of non randomized studies in meta-analyses. 2011, http://www.ohri.ca/programs/clinical_epidemiology/oxford.htm. Accessed Novenber 21, 2012Google Scholar
  23. DerSimonian R, Laird N: Meta-analysis in clinical trials. Control Clin Trials. 1986, 7 (3): 177-188. 10.1016/0197-2456(86)90046-2.View ArticlePubMedGoogle Scholar
  24. Greenland S, Longnecker MP: Methods for trend estimation from summarized dose–response data, with applications to meta-analysis. Am J Epidemiol. 1992, 135 (11): 1301-1309.PubMedGoogle Scholar
  25. Orsini N, Bellocco R, Greenland S: Generalized least squares for trend estimation of summarized dose–response data. Stata J. 2006, 6 (1): 40-57.Google Scholar
  26. Larsson SC, Orsini N, Wolk A: Vitamin B6 and risk of colorectal cancer: a meta-analysis of prospective studies. JAMA. 2010, 303 (11): 1077-1083. 10.1001/jama.2010.263.View ArticlePubMedGoogle Scholar
  27. Harrell FE, Lee KL, Pollock BG: Regression models in clinical studies: determining relationships between predictors and response. J Natl Cancer Inst. 1988, 80 (15): 1198-1202. 10.1093/jnci/80.15.1198.View ArticlePubMedGoogle Scholar
  28. Higgins JP, Thompson SG, Deeks JJ, Altman DG: Measuring inconsistency in meta-analyses. BMJ. 2003, 327 (7414): 557-560. 10.1136/bmj.327.7414.557.View ArticlePubMedPubMed CentralGoogle Scholar
  29. Egger M, Davey Smith G, Schneider M, Minder C: Bias in meta-analysis detected by a simple, graphical test. BMJ. 1997, 315 (7109): 629-634. 10.1136/bmj.315.7109.629.View ArticlePubMedPubMed CentralGoogle Scholar
  30. Peters JL, Sutton AJ, Jones DR, Abrams KR, Rushton L: Contour-enhanced meta-analysis funnel plots help distinguish publication bias from other causes of asymmetry. J Clin Epidemiol. 2008, 61 (10): 991-996. 10.1016/j.jclinepi.2007.11.010.View ArticlePubMedGoogle Scholar
  31. Duval S, Tweedie R: Trim and fill: a simple funnel-plot-based method of testing and adjusting for publication bias in meta-analysis. Biometrics. 2000, 56 (2): 455-463. 10.1111/j.0006-341X.2000.00455.x.View ArticlePubMedGoogle Scholar
  32. Thiebaut AC, Chajes V, Gerber M, Boutron-Ruault MC, Joulin V, Lenoir G, Berrino F, Riboli E, Benichou J, Clavel-Chapelon F: Dietary intakes of omega-6 and omega-3 polyunsaturated fatty acids and the risk of breast cancer. Int J Cancer. 2009, 124: 924-931. 10.1002/ijc.23980. 2008/11/28 ednView ArticlePubMedGoogle Scholar
  33. Sczaniecka AK, Brasky TM, Lampe JW, Patterson RE, White E: Dietary intake of specific fatty acids and breast cancer risk among postmenopausal women in the VITAL cohort. Nutrition and cancer. 2012, 64 (8): 1131-1142. 10.1080/01635581.2012.718033.View ArticlePubMedPubMed CentralGoogle Scholar
  34. Chajes V, Hulten K, Van Kappel AL, Winkvist A, Kaaks R, Hallmans G, Lenner PG, Riboli E: Fatty-acid composition in serum phospholipids and risk of breast cancer: an incident case–control study in Sweden. Int J Cancer. 1999, 83 (5): 585-590. 10.1002/(SICI)1097-0215(19991126)83:5<585::AID-IJC2>3.0.CO;2-Z.View ArticlePubMedGoogle Scholar
  35. Wirfalt E, Mattisson I, Gullberg B, Johansson U, Olsson H, Berglund G: Postmenopausal breast cancer is associated with high intakes of (omega)6 fatty acids (Sweden). Cancer Causes Control. 2002, 13 (10): 883-893. 10.1023/A:1021922917489.View ArticlePubMedGoogle Scholar
  36. Takata Y, King IB, Neuhouser ML, Schaffer S, Barnett M, Thornquist M, Peters U, Goodman GE: Association of serum phospholipid fatty acids with breast cancer risk among postmenopausal cigarette smokers. Cancer Cause Control. 2009, 20 (4): 497-504. 10.1007/s10552-009-9314-2.View ArticleGoogle Scholar
  37. Simopoulos AP: The Mediterranean diets: What is so special about the diet of Greece? The scientific evidence. J Nutr. 2001, 131 (11 SUPPL): 3065S-3073S.PubMedGoogle Scholar
  38. Signori C, DuBrock C, Richie JP, Prokopczyk B, Demers LM, Hamilton C, Hartman TJ, Liao J, El-Bayoumy K, Manni A: Administration of omega-3 fatty acids and Raloxifene to women at high risk of breast cancer: interim feasibility and biomarkers analysis from a clinical trial. Eur J Clin Nutr. 2012, 66 (8): 878-884. 10.1038/ejcn.2012.60.View ArticlePubMedGoogle Scholar
  39. Reader J, Holt D, Fulton A: Prostaglandin E2 EP receptors as therapeutic targets in breast cancer. Cancer Metastasis Rev. 2011, 30 (3–4): 449-463.View ArticlePubMedPubMed CentralGoogle Scholar
  40. Hopkins GJ, Kennedy TG, Carroll KK: Polyunsaturated fatty acids as promoters of mammary carcinogenesis induced in Sprague–Dawley rats by 7,12-dimethylbenz[a]anthracene. J Natl Cancer Inst. 1981, 66 (3): 517-522.PubMedGoogle Scholar
  41. Grammatikos SI, Subbaiah PV, Victor TA, Miller WM: n-3 and n-6 fatty acid processing and growth effects in neoplastic and non-cancerous human mammary epithelial cell lines. Br J Cancer. 1994, 70 (2): 219-227. 10.1038/bjc.1994.283.View ArticlePubMedPubMed CentralGoogle Scholar
  42. Rovito D, Giordano C, Vizza D, Plastina P, Barone I, Casaburi I, Lanzino M, De Amicis F, Sisci D, Mauro L, Aquila S, Catalano S, Bonofiglio D, Andò S: Omega-3 PUFA ethanolamides DHEA and EPEA induce autophagy through PPAR(gamma) activation in MCF-7 breast cancer cells. J Cell Physiol. 2013, 228 (6): 1314-1322. 10.1002/jcp.24288.View ArticlePubMedGoogle Scholar
  43. Rose DP, Connolly JM: Omega-3 fatty acids as cancer chemopreventive agents. Pharmacol Ther. 1999, 83 (3): 217-244. 10.1016/S0163-7258(99)00026-1.View ArticlePubMedGoogle Scholar
  44. Obata T, Nagakura T, Masaki T, Maekawa K, Yamashita K: Eicosapentaenoic acid inhibits prostaglandin D2 generation by inhibiting cyclo-oxygenase-2 in cultured human mast cells. Clin Exp Allergy. 1999, 29 (8): 1129-1135. 10.1046/j.1365-2222.1999.00604.x.View ArticlePubMedGoogle Scholar
  45. Hamid R, Singh J, Reddy BS, Cohen LA: Inhibition by dietary menhaden oil of cyclooxygenase-1 and 2 in N-nitrosomethylurea-induced rat mammary tumors. Int J Oncol. 1999, 14 (3): 523-528.PubMedGoogle Scholar
  46. Pietinen P, Vartiainen E, Seppanen R, Aro A, Puska P: Changes in diet in Finland from 1972 to 1992: Impact on coronary heart disease risk. Prev Med. 1996, 25 (3): 243-250. 10.1006/pmed.1996.0053.View ArticlePubMedGoogle Scholar
  47. Nettleton JA: Omega-3-fatty-acids - comparison of plant and seafood sources in human-nutrition. J Am Diet Assoc. 1991, 91 (3): 331-337.PubMedGoogle Scholar
  48. Pre-publication history

    1. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/14/105/prepub

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